Department of Biotechnology, National Kaohsiung Normal University, 116 Ho-Ping First Rd., Lin-Ya 802, Kaohsiung, Taiwan.
Toxicol In Vitro. 2010 Jun;24(4):1197-202. doi: 10.1016/j.tiv.2010.02.011. Epub 2010 Feb 13.
Para-phenylenediamine (p-PD), a suspected carcinogen, is a component of permanent hair dyes. In this study we examined the mechanism of cytotoxicity and genotoxicity in Mardin-Darby canine kidney cells (MDCK)-treated with p-PD. Our results showed that p-PD decreased cell viability in a dose- and time-dependent manner. In addition, p-PD induced DNA damage was confirmed by the comet and TUNEL assays. Pre-treatment of MDCK cells with antioxidants vitamin C or E significantly inhibited p-PD induced cytotoxicity and reactive oxygen species (ROS) generation. Furthermore, p-PD induced apoptosis through activated initiator caspase-8 and -9, and effector caspase-3/7. Based on these results, we suggested that p-PD induce apoptosis which was mediated with caspase-8, caspase-9 and caspase-3/7 activation via the involvement of ROS.
对苯二胺（p-PD）是一种疑似致癌物质，是永久性染发剂的成分之一。在这项研究中，我们研究了 p-PD 处理下马尔丁-达比犬肾细胞（MDCK）的细胞毒性和遗传毒性的机制。结果表明，p-PD 呈剂量和时间依赖性地降低细胞活力。此外，彗星和 TUNEL 检测证实 p-PD 诱导了 DNA 损伤。抗氧化剂维生素 C 或 E 预处理 MDCK 细胞可显著抑制 p-PD 诱导的细胞毒性和活性氧（ROS）生成。此外，p-PD 通过激活起始半胱天冬酶-8 和 -9 以及效应半胱天冬酶-3/7 诱导细胞凋亡。基于这些结果，我们认为 p-PD 通过 ROS 的参与，通过激活 caspase-8、caspase-9 和 caspase-3/7 诱导细胞凋亡。
