Pannacci Marilou, Lucini Valeria, Colleoni Federica, Martucci Cataldo, Grosso Silvia, Sacerdote Paola, Scaglione Francesco
Department of Pharmacology, Chemotherapy and Toxicology, Faculty of Medicine, University of Milan, via Vanvitelli 32, 20129 Milan, Italy.
Brain Behav Immun. 2006 Nov;20(6):546-51. doi: 10.1016/j.bbi.2005.11.007. Epub 2006 Feb 15.
The effect of Panax ginseng C.A. Meyer G115 on inflammatory cytokine production and toll-like receptor 4 (TLR4) RNA expression was examined in mice during 4 weeks of swimming stress. Mice were assigned to four groups: (1) control (no exercise); (2) control-G115 (25 mg/kg/day p.o.); (3) stress (kept swimming for 60 min daily); and (4) stress-G115 (25 mg/kg/day p.o. and kept swimming for 60 min daily). Peritoneal macrophages were collected at rest each week. RNA was extracted and processed for real-time PCR. An aliquot of macrophages was lipopolysaccharide (LPS)-stimulated for TNF-alpha and IL-1beta production. Different expression patterns between untreated and treated groups, and between TLR2 and TLR4 were found. High levels of TLR4 expression in the control-G115 group were detectable significantly at the first, and at the second week (P<.01 and P<.001, respectively). In the stress group, TLR4 showed a peak at the first week (P<.001 vs. controls) and then decreased gradually. In the stress-G115 group, the levels of TLR4 expression increased gradually at the second week (P<.001 vs. controls) with a peak at the third week (P<.001). Levels of TLR4 expression at the fourth week had returned to the basal level. Levels of TLR2 expression were not affected by treatment in all groups. A significant increase of LPS-stimulated IL-1beta and TNF-alpha concentrations was present in trained animals with similar patterns of TLR4 expression. These results support the hypothesis that enhancement of the production of pro-inflammatory cytokine can be linked to an increased expression of TLR4 on macrophages. Moreover, G115 increases the expression of TLR4 and the release of cytokines with a different pattern compared to the stressed alone group.
在为期4周的游泳应激期间，研究了人参C.A. 迈耶G115对小鼠炎性细胞因子产生及Toll样受体4（TLR4）RNA表达的影响。将小鼠分为四组：（1）对照组（不运动）；（2）对照-G115组（口服25 mg/kg/天）；（3）应激组（每天持续游泳60分钟）；（4）应激-G115组（口服25 mg/kg/天且每天持续游泳60分钟）。每周在小鼠休息时收集腹腔巨噬细胞。提取RNA并进行实时PCR处理。取一部分巨噬细胞用脂多糖（LPS）刺激以产生肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）。发现未处理组与处理组之间以及TLR2和TLR4之间存在不同的表达模式。对照-G115组在第一周和第二周可检测到高水平的TLR4表达（分别为P<0.01和P<0.001）。在应激组中，TLR4在第一周出现峰值（与对照组相比P<0.001），然后逐渐下降。在应激-G115组中，TLR4表达水平在第二周逐渐升高（与对照组相比P<0.001），在第三周达到峰值（P<0.001）。第四周TLR4表达水平已恢复到基础水平。所有组中TLR2的表达水平均不受处理影响。在具有相似TLR4表达模式的训练动物中，LPS刺激的IL-1β和TNF-α浓度显著增加。这些结果支持以下假设：促炎细胞因子产生的增强可能与巨噬细胞上TLR4表达的增加有关。此外，与单独应激组相比，G115以不同模式增加TLR4的表达和细胞因子的释放。
