Neal R A, Johnson P
Acta Trop. 1977 Mar;34(1):87-96.
The immunization of mice with killed epimastigotes or trypomastigotes with saponin SPL as adjuvant was challenged by inoculation of blood or metacyclic trypomastigotes of T. cruzi. Immunogenic preparations were obtained by freeze-thawing of formalin treatment. The protection was effective against homologous and heterologous challenge, and lasted up to 12 weeks after the last immunization. The immunization was also effective against metacyclic trypomastigote challenge. The immunogenicity of the killed T. cruzi suspension was retained after freeze-drying.
用皂角苷SPL作为佐剂，用灭活的上鞭毛体或锥鞭毛体对小鼠进行免疫接种，然后通过接种克氏锥虫的血液或循环后期锥鞭毛体进行攻毒。通过对经福尔马林处理的样本进行冻融来获得免疫原性制剂。这种保护对同源和异源攻毒均有效，并且在最后一次免疫后可持续长达12周。该免疫接种对循环后期锥鞭毛体攻毒也有效。冻干后，灭活的克氏锥虫悬液仍保留免疫原性。
