McHardy H
Tropenmed Parasitol. 1977 Mar;28(1):11-6.
Groups of CD-1 mice were immunized with a vaccine prepared from freeze-thawed cultured epimastigotes of Trypanosoma cruzi, strain Y, with or without saponin as adjuvant. The vaccine was injected by the subcutaneous (s.c.) or intraperitoneal (i.p.) route. Immunized and control mice were challenged with homologous blood trypomastigotes by the s.c. or i.p. route and the former route was found to produce a far more severe and uniform infection in both control and immunized mice. Subcutaneous challenge of vaccinated mice demonstrated an improvement in the protective effect of the vaccine when saponin was included, but following i.p. challenge equally good protection was achieved with or without saponin. Thirty-nine of 40 immunized mice challenged i.p. survived the infection. A strong correlation was shown between the size of both immunizing and challenge inocula and the protective effect of the vaccine. Immunized mice which survived challenge were resistant to reinfection, but all were shown to carry persistent sub-patent parasitaemia.
将几组CD-1小鼠用由经冻融处理的克氏锥虫Y株培养的无鞭毛体制备的疫苗进行免疫，该疫苗添加或不添加皂苷作为佐剂。疫苗通过皮下（s.c.）或腹腔内（i.p.）途径注射。免疫小鼠和对照小鼠通过皮下或腹腔内途径用同源血液型锥鞭毛体进行攻击，发现前一种途径在对照小鼠和免疫小鼠中均产生更为严重且一致的感染。对接种疫苗的小鼠进行皮下攻击表明，当加入皂苷时疫苗的保护效果有所改善，但在腹腔内攻击后，无论有无皂苷均能获得同样良好的保护效果。40只经腹腔内攻击的免疫小鼠中有39只在感染中存活。免疫接种和攻击接种物的大小与疫苗的保护效果之间显示出强烈的相关性。在攻击中存活的免疫小鼠对再次感染具有抗性，但所有小鼠均显示携带持续的亚临床寄生虫血症。
