Perry Paul A, Fitzgerald Melissa A, Gilbert Robert G
Key Centre for Polymer Colloids, School of Chemistry F11, University of Sydney, NSW 2006 Australia.
Biomacromolecules. 2006 Feb;7(2):521-30. doi: 10.1021/bm0507711.
The diffusion coefficients of dextran probes of various molecular weights in starch solutions over a wide concentration range were carried out using fluorescent recovery after photobleaching (FRAP), combined with a confocal microscope and tracer probe diffusion. The technique is simple to implement and can be carried out using increasingly common microscopy apparatus, giving access to a wide variety of new structural and kinetic information. The data can be rationalized in terms of the effects of probe molecular weight and on matrix starch concentration and structure. This provides a new tool to investigate the behavior of systems where starch is an ingredient that contributes to the processing and textural properties of food.
在很宽的浓度范围内，使用光漂白后荧光恢复（FRAP）技术，结合共聚焦显微镜和示踪探针扩散，测定了各种分子量的葡聚糖探针在淀粉溶液中的扩散系数。该技术易于实施，可使用越来越常见的显微镜设备进行，从而获得各种新的结构和动力学信息。这些数据可以根据探针分子量以及基质淀粉浓度和结构的影响进行合理分析。这为研究淀粉作为影响食品加工和质地特性的成分的体系行为提供了一种新工具。
