Albro Michael B, Rajan Vikram, Li Roland, Hung Clark T, Ateshian Gerard A
Department of Biomedical Engineering, Columbia University, New York.
Cell Mol Bioeng. 2009 Sep 1;2(3):295-305. doi: 10.1007/s12195-009-0076-4.
This study reports experimental measurements of solute diffusivity and partition coefficient for various solute concentrations and gel porosities, and proposes novel constitutive relations to describe these observed values. The longer-term aim is to explore the theoretical ramifications of accommodating variations in diffusivity and partition coefficient with solute concentration and tissue porosity, and investigate whether they might suggest novel mechanisms not previously recognized in the field of solute transport in deformable porous media. The study implements a model transport system of agarose hydrogels to investigate the effect of solute concentration and hydrogel porosity on the transport of dextran polysaccharides. The proposed phenomenological constitutive relations are shown to provide better fits of experimental results than prior models proposed in the literature based on the microstructure of the gel. While these constitutive models were developed for the transport of dextran in agarose hydrogels, it is expected that they may also be applied to the transport of similar molecular weight solutes in other porous media. This quantification can assist in the application of biophysical models that describe biological transport in deformable tissues, as well as the cell cytoplasm.
本研究报告了针对各种溶质浓度和凝胶孔隙率的溶质扩散率和分配系数的实验测量结果，并提出了新的本构关系来描述这些观测值。长期目标是探讨考虑扩散率和分配系数随溶质浓度和组织孔隙率变化的理论影响，并研究它们是否可能暗示在可变形多孔介质中溶质传输领域以前未被认识的新机制。该研究采用了琼脂糖水凝胶的模型传输系统，以研究溶质浓度和水凝胶孔隙率对葡聚糖多糖传输的影响。结果表明，所提出的唯象本构关系比文献中基于凝胶微观结构提出的先前模型能更好地拟合实验结果。虽然这些本构模型是为葡聚糖在琼脂糖水凝胶中的传输而开发的，但预计它们也可应用于其他多孔介质中类似分子量溶质的传输。这种量化有助于应用描述可变形组织以及细胞质中生物传输的生物物理模型。
