Fujii T
Kyoto Municipal Jr. College of Nursing, Japan.
Brain Res. 1991 Feb 1;540(1-2):224-8. doi: 10.1016/0006-8993(91)90511-s.
Percent reduction profiles of cytochromes (cyt.) aa3, b and c were investigated in bloodless guinea pig hippocampal brain slices of 400, 600 and 800 microns in thickness ranging in temperature from 22 to 37 degrees C. The extent of the percent reduction of cytochromes was compared with the generation of orthodromic potentials elicited by the stimulation of the stratum radiatum, and the cessation of the potentials was found to be correlated with the extent of the percent reduction of the cytochromes. In the case of 400 microns slices, they were found to be in normoxia both from the extent of the percent reduction levels of cytochromes and from the generation of orthodromic responses over a range in temperature. In the case of 600 microns slices, those incubated under temperatures of 22 to 32 degrees C were not in hypoxia from the levels of cytochrome reduction and the production of a field potential. However, slices at 37 degrees C were in hypoxia because of cyt. c levels approached those of cyt. b and the orthodromic response was suppressed. In 800 microns slices, those at 22-27 degrees C were in normoxia; however, slices maintained at 32-37 degrees C were in hypoxia because the levels of cyt. c reduction closely approximated those of cyt. b at 32 degrees C whereas those of cyt. aa3, b and c were almost the same as at 37 degrees C. Moreover, the orthodromic field potential was not evoked.
在厚度为400、600和800微米的无血豚鼠海马脑片中，研究了细胞色素（cyt.）aa3、b和c的百分还原曲线，温度范围为22至37摄氏度。将细胞色素的百分还原程度与放射层刺激引发的顺向电位的产生进行了比较，发现电位的停止与细胞色素的百分还原程度相关。对于400微米厚的脑片，从细胞色素百分还原水平的程度以及在一定温度范围内顺向反应的产生来看，发现它们处于常氧状态。对于600微米厚的脑片，在22至32摄氏度下孵育的脑片，从细胞色素还原水平和场电位的产生来看，没有处于缺氧状态。然而，37摄氏度下的脑片处于缺氧状态，因为细胞色素c的水平接近细胞色素b的水平，顺向反应受到抑制。在800微米厚的脑片中，22 - 27摄氏度下的脑片处于常氧状态；然而，保持在32 - 37摄氏度的脑片处于缺氧状态，因为在32摄氏度时细胞色素c的还原水平与细胞色素b的还原水平非常接近，而在37摄氏度时细胞色素aa3、b和c的还原水平几乎相同。此外，没有诱发顺向场电位。
