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一种用于同时记录体外脑片标本电活动和分光光度信号的方法。

A method for simultaneous recording of electrical activities and spectrophotometric signals from brain slice preparations in vitro.

作者信息

Fujii T, Toita K

机构信息

Kyoto Municipal Jr. College of Nursing, Japan.

出版信息

Pflugers Arch. 1991 Sep;419(2):205-8. doi: 10.1007/BF00373008.

DOI:10.1007/BF00373008
PMID:1961689
Abstract

Organ spectrophotometry has been applied to analyze cytochrome redox changes in brain slice preparations. An interface-chamber method for maintaining metabolism of brain slice tissues was devised to reduce noise on recording traces of spectrophotometric signals, and then used for continuous monitoring and simultaneous recording of electrical and optical signals from brain slices. With this method, the noise level during the recording of redox states of cytochromes was decreased to 0.0004 A unit.

摘要

器官分光光度法已被应用于分析脑片制剂中的细胞色素氧化还原变化。设计了一种用于维持脑片组织代谢的界面室方法，以减少分光光度信号记录轨迹上的噪声，然后用于连续监测和同步记录来自脑片的电信号和光信号。使用这种方法，细胞色素氧化还原状态记录期间的噪声水平降至0.0004吸光度单位。

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本文引用的文献

1
Correlation of field potentials with redox-states of cytochromes recorded from the olfactory cortical slice of guinea pig.豚鼠嗅觉皮质切片记录的场电位与细胞色素氧化还原状态的相关性。
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在体外培养的豚鼠嗅觉皮层中观察到的电活动。
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Gas-blow and medium-flow method for stable recording of electrical activities in the sliced mammalian cerebral tissue in vitro.
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Correlation between adaptability to hypoxia and thickness of guinea pig olfactory cortical slice in vitro.
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Profiles of percent reduction of cytochromes in guinea pig hippocampal brain slices in vitro.豚鼠海马脑片体外细胞色素减少百分比概况。
Brain Res. 1991 Feb 1;540(1-2):224-8. doi: 10.1016/0006-8993(91)90511-s.
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Effects of cooling on guinea pig olfactory cortex maintained in vitro.冷却对体外培养的豚鼠嗅觉皮层的影响。
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