Orihuela Pedro A, Ishiyama Victor
Laboratorio de Reproducción y Biología del Desarrollo, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos, Lima, Perú.
Contraception. 2006 Mar;73(3):307-10. doi: 10.1016/j.contraception.2005.08.015. Epub 2005 Oct 21.
We examined whether the aqueous extract of Erythrina falcata, reputed to be a contraceptive in Peruvian folklore, could prevent pregnancy in the mouse.
Female mice on Day 1 of pregnancy were given aqueous extract of E. falcata or tap water (control) orally for 4 days. On Day 4 of pregnancy, animals were killed and the embryos were flushed from oviducts and uterus to examine their developmental stage, cell number, mitotic index and micronuclei frequency. Other mice were killed on Day 12 of pregnancy to determine the number of implantation sites.
Ingestion of E. falcata diminished the percentage of embryos that progressed to blastocyst stage, reduced the cell number and mitotic index, and increased the micronuclei frequency of early embryos. The number of implantation sites was also reduced in females treated with E. falcata.
The aqueous extract of E. falcata, ingested during early pregnancy, disturbs preimplantation embryo development and implantation in the mouse. These results provide the first experimental evidence of the contraceptive properties of the aqueous extract of E. falcata.
我们研究了在秘鲁民间传说中被认为具有避孕作用的镰叶刺桐水提取物是否能防止小鼠怀孕。
怀孕第1天的雌性小鼠口服镰叶刺桐水提取物或自来水（对照），持续4天。在怀孕第4天，处死动物，从输卵管和子宫中冲洗出胚胎，检查其发育阶段、细胞数量、有丝分裂指数和微核频率。其他小鼠在怀孕第12天处死，以确定着床部位的数量。
摄入镰叶刺桐水提取物会降低发育至囊胚阶段的胚胎百分比，减少细胞数量和有丝分裂指数，并增加早期胚胎的微核频率。用镰叶刺桐水提取物处理的雌性小鼠的着床部位数量也减少了。
怀孕早期摄入镰叶刺桐水提取物会干扰小鼠着床前胚胎的发育和着床。这些结果为镰叶刺桐水提取物的避孕特性提供了首个实验证据。
