Jana Barbara, Kucharski Jan, Dzienis Anna, Deptuła Katarzyna
Division of Reproductive Endocrinology and Pathophysiology, Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Tuwima 10, 10-747 Olsztyn, Poland.
Anim Reprod Sci. 2007 Jan;97(1-2):137-50. doi: 10.1016/j.anireprosci.2006.01.002. Epub 2006 Feb 13.
The aim of this study was to determine the prostaglandins (PGs) production and ovarian function in gilts after intrauterine infusions of 10(6) and 10(9) colony-forming units (cfu)/ml of Escherichia coli (E. coli). In Experiments 1 and 2, 30 ml of saline or 30 ml of E. coli suspension containing 10(6) or 10(9)cfu/ml, were infused once into each uterine horn in three groups of gilts on day 3 of the estrous cycle, respectively. In Experiment 1, 17 days after treatment it was revealed that inoculation of E. coli 10(9)cfu/ml induced severe acute or subacute endometritis while 10(6)cfu of E. coli evoked moderate acute endometritis or resulted in no inflammatory changes. In the gilts receiving 10(9)cfu/ml of E. coli, the concentration of 13,14-dihydro-15-keto-PGF(2)alpha in blood from the jugular vein was elevated (P<0.05-0.001) compared to concentration in the gilts inoculated with 10(6)cfu on days 8-17 after treatment. Both the E. coli-treated groups had a lower (P<0.05, P<0.01) progesterone plasma level from days 10 to 14 after administration than the control group. On day 17 of the study, infusion of E. coli 10(9)cfu/ml, in comparison to 10(6)cfu, resulted in the greater (P<0.001) content of PGE(2) in the myometrium. The content of both PGs in the endometrium as well as PGF(2alpha) in the myometrium of gilts-treated with 10(9)cfu/ml of E. coli was lower (P<0.001) than in gilts-treated with 10(6)cfu of bacteria. Newly formed corpora lutea were found in the gilts infused with 10(6), but not those infused with 10(9)cfu/ml of E. coli on day 17 after infusion. On day 8 of the study (Experiment 2), the blood from utero-ovarian vein of the gilts-treated with 10(9)cfu/ml of bacteria had a higher (P<0.05) PGF(2alpha) level and lower (P<0.001) PGE(2) level than following infusion of E. coli 10(6)cfu/ml. Also on day 8 of the study, the content of PGE(2) in the endometrium, both the PGs in the myometrium as well as cyclooxygenase-2 in the endometrium and myometrium was greater (P<0.01, P<0.001) after applying 10(9)cfu/ml than 10(6)cfu/ml of E. coli. These results indicate that intrauterine infusions of 10(6) or 10(9)cfu/ml of E. coli lead to the development of inflammatory states of different intensities which is connected with different PGF(2alpha) and PGE(2) production and function of ovaries.
本研究的目的是确定在发情周期第3天向后备母猪子宫内注入每毫升含10⁶和10⁹菌落形成单位（cfu）的大肠杆菌（E. coli）后，后备母猪体内前列腺素（PGs）的产生情况及卵巢功能。在实验1和实验2中，分别向三组后备母猪的每个子宫角一次性注入30毫升生理盐水或30毫升含每毫升10⁶或10⁹cfu的大肠杆菌悬液。在实验1中，处理后17天发现，注入每毫升10⁹cfu的大肠杆菌会引发严重的急性或亚急性子宫内膜炎，而注入10⁶cfu的大肠杆菌会引起中度急性子宫内膜炎或不产生炎症变化。在接受每毫升10⁹cfu大肠杆菌处理的后备母猪中，与在处理后第8 - 17天接种每毫升10⁶cfu大肠杆菌的后备母猪相比，颈静脉血中13,14 - 二氢 - 15 - 酮 - PGF₂α的浓度升高（P<0.05 - 0.001）。在给药后第10至14天，两个大肠杆菌处理组的血浆孕酮水平均低于对照组（P<0.05，P<0.01）。在研究的第17天，与注入每毫升10⁶cfu的大肠杆菌相比，注入每毫升10⁹cfu的大肠杆菌导致子宫肌层中PGE₂的含量更高（P<0.001）。在接受每毫升10⁹cfu大肠杆菌处理的后备母猪的子宫内膜中，两种PGs以及子宫肌层中的PGF₂α的含量均低于接受每毫升10⁶cfu大肠杆菌处理的后备母猪（P<0.001）。在注入后第17天，在注入每毫升10⁶cfu大肠杆菌的后备母猪中发现了新形成的黄体，但在注入每毫升10⁹cfu大肠杆菌的后备母猪中未发现。在研究的第8天（实验2），与注入每毫升10⁶cfu大肠杆菌相比，接受每毫升10⁹cfu大肠杆菌处理的后备母猪子宫 - 卵巢静脉血中的PGF₂α水平更高（P<0.05），PGE₂水平更低（P<0.001）。同样在研究的第8天，注入每毫升10⁹cfu大肠杆菌后，子宫内膜中PGE₂的含量、子宫肌层中两种PGs以及子宫内膜和子宫肌层中环氧合酶 - 2的含量均高于注入每毫升10⁶cfu大肠杆菌后（P<0.01，P<0.001）。这些结果表明，向子宫内注入每毫升10⁶或10⁹cfu的大肠杆菌会导致不同强度的炎症状态的发展，这与不同的PGF₂α和PGE₂产生以及卵巢功能有关。
