Mierzejewski Karol, Stryiński Robert, Bogacka Iwona, Golubska Monika, Carrera Mónica, Kurzynska Aleksandra
Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.
Department of Biochemistry, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.
Front Mol Biosci. 2025 Jan 7;11:1523098. doi: 10.3389/fmolb.2024.1523098. eCollection 2024.
Chronic inflammation caused by infections has a significant negative impact on the reproductive system and impairs fertility. The corpus luteum (CL) plays a central role not only in regulating the ovary cycle, but also in implantation of the embryo and maintenance of early pregnancy through the secretion of progesterone. Understanding the intricate interplay between inflammatory processes and reproductive organ's function is crucial for the development of effective therapeutic strategies to alleviate reproductive disorders and improve fertility.
The aim of this study was to determine the in vitro effects of lipopolysaccharide (LPS) on the proteomic profile of the porcine CL in the mid-luteal phase of the estrous cycle using LC-MS/MS analysis. The CL slices were incubated in the presence of LPS for 24 h.
We identified 12 differentially regulated proteins after treatment with LPS (7 of them were upregulated, while 5 were downregulated). The analysis showed that these proteins are involved in processes such as glucose metabolism, the tricarboxylic acid cycle (TCA), detoxification processes as well as steroid biosynthesis in the CL. Moreover, we demonstrated that LPS decreases glucose levels and increases progesterone levels in the CL.
These findings suggest that LPS modulates key metabolic pathways in the CL, potentially impacting its functional activity.
感染引起的慢性炎症对生殖系统有重大负面影响并损害生育能力。黄体（CL）不仅在调节卵巢周期中起核心作用，还通过分泌孕酮在胚胎着床和早期妊娠维持中发挥关键作用。了解炎症过程与生殖器官功能之间的复杂相互作用对于开发有效的治疗策略以缓解生殖障碍和提高生育能力至关重要。
本研究的目的是使用液相色谱 - 串联质谱（LC - MS/MS）分析确定脂多糖（LPS）对发情周期中期猪黄体蛋白质组学图谱的体外影响。将黄体切片在LPS存在下孵育24小时。
在用LPS处理后，我们鉴定出12种差异调节蛋白（其中7种上调，5种下调）。分析表明，这些蛋白质参与黄体中的葡萄糖代谢、三羧酸循环（TCA）、解毒过程以及类固醇生物合成等过程。此外，我们证明LPS降低了黄体中的葡萄糖水平并增加了孕酮水平。
这些发现表明LPS调节黄体中的关键代谢途径，可能影响其功能活性。
