痘苗病毒4c蛋白和A型包含体蛋白是细胞内成熟病毒颗粒的特异性标志物。
The vaccinia virus 4c and A-type inclusion proteins are specific markers for the intracellular mature virus particle.
作者信息
Ulaeto D, Grosenbach D, Hruby D E
机构信息
Chemical & Biological Defence Establishment, Porton Down, Salisbury, United Kingdom.
出版信息
J Virol. 1996 Jun;70(6):3372-7. doi: 10.1128/JVI.70.6.3372-3377.1996.
Abstract
Gel analysis of vaccinia virus particles purified by buoyant [correction of bouyant] density demonstrates a protein with an estimated molecular mass of 59 kDa, which is apparently restricted to the intracellular mature virion (IMV) form. Western blotting (immunoblotting) and immunoprecipitation procedures identify the protein as the vaccinia virus 4c protein, which facilitates occlusion of poxvirus particles within cowpox cytoplasmic inclusions. Western blotting procedures also identify the truncated A-type inclusion protein of vaccinia virus as a specific marker for IMV particles. Kinetic analyses of virion maturation and 4c production suggest that peak enveloped virion production occurs before peak IMV production in the virus replication cycle and that 4c production is concomitant with maturation of IMV. The implications for a distinct and evolutionarily conserved function of IMV in viral pathogenesis are discussed.
摘要
通过浮力密度纯化的痘苗病毒颗粒的凝胶分析显示，有一种估计分子量为59 kDa的蛋白质，它显然只存在于细胞内成熟病毒粒子（IMV）形式中。蛋白质印迹法（免疫印迹法）和免疫沉淀程序确定该蛋白质为痘苗病毒4c蛋白，它有助于痘病毒颗粒在牛痘细胞质内含物中被包裹。蛋白质印迹法程序还确定痘苗病毒截短的A型内含物蛋白是IMV颗粒的特异性标志物。病毒粒子成熟和4c产生的动力学分析表明，在病毒复制周期中，有包膜病毒粒子的产量峰值出现在IMV产量峰值之前，并且4c的产生与IMV的成熟同时发生。本文讨论了IMV在病毒发病机制中独特且进化保守功能的意义。
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本文引用的文献
1
The vaccinia virus 42-kDa envelope protein is required for the envelopment and egress of extracellular virus and for virus virulence.痘苗病毒42-kDa包膜蛋白是细胞外病毒的包膜形成与释放以及病毒毒力所必需的。
Virology. 1993 Jun;194(2):627-37. doi: 10.1006/viro.1993.1302.
2
Dissociation of progeny vaccinia virus from the cell membrane is regulated by a viral envelope glycoprotein: effect of a point mutation in the lectin homology domain of the A34R gene.子代痘苗病毒从细胞膜的解离受一种病毒包膜糖蛋白调控：A34R基因凝集素同源结构域点突变的影响。
J Virol. 1993 Jun;67(6):3319-25. doi: 10.1128/JVI.67.6.3319-3325.1993.
3
Assembly of vaccinia virus: role of the intermediate compartment between the endoplasmic reticulum and the Golgi stacks.痘苗病毒的组装：内质网与高尔基体堆栈之间中间区室的作用。
J Cell Biol. 1993 May;121(3):521-41. doi: 10.1083/jcb.121.3.521.
4
Characterization of the gene encoding the A-type inclusion protein of camelpox virus and sequence comparison with other orthopoxviruses.骆驼痘病毒A 型包涵体蛋白编码基因的特性分析及其与其他正痘病毒的序列比较。
J Gen Virol. 1993 Aug;74 ( Pt 8):1679-84. doi: 10.1099/0022-1317-74-8-1679.
5
Deletion of the vaccinia virus B5R gene encoding a 42-kilodalton membrane glycoprotein inhibits extracellular virus envelope formation and dissemination.编码一种42千道尔顿膜糖蛋白的痘苗病毒B5R基因的缺失会抑制细胞外病毒包膜的形成和传播。
J Virol. 1993 Aug;67(8):4732-41. doi: 10.1128/JVI.67.8.4732-4741.1993.
6
Assembly of vaccinia virus: the second wrapping cisterna is derived from the trans Golgi network.痘苗病毒的组装：第二个包裹池源自反式高尔基体网络。
J Virol. 1994 Jan;68(1):130-47. doi: 10.1128/JVI.68.1.130-147.1994.
7
Vaccinia virus gene A36R encodes a M(r) 43-50 K protein on the surface of extracellular enveloped virus.痘苗病毒基因A36R在细胞外被膜病毒表面编码一种分子量为43 - 50K的蛋白质。
Virology. 1994 Oct;204(1):376-90. doi: 10.1006/viro.1994.1542.
8
Significance of extracellular enveloped virus in the in vitro and in vivo dissemination of vaccinia.细胞外被膜病毒在痘苗病毒体外和体内传播中的意义。
J Gen Virol. 1980 Sep;50(1):89-100. doi: 10.1099/0022-1317-50-1-89.
9
A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.一种用于检测聚丙烯酰胺凝胶中氚标记蛋白质和核酸的胶片检测方法。
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10
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Virology. 1972 Nov;50(2):579-92. doi: 10.1016/0042-6822(72)90409-6.
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