Inoue N, Harada S, Miyasaka N, Oya A, Yanagi K
Department of Virology and Rickettsiology, National Institute of Health, Tokyo, Japan.
J Infect Dis. 1991 Jul;164(1):22-8. doi: 10.1093/infdis/164.1.22.
To examine if Epstein-Barr virus (EBV) infection is associated with the two autoimmune diseases, Sjögren's syndrome and rheumatoid arthritis, IgG antibody titers of sera from patients with the disorders were evaluated for five constituents of EBV nuclear antigens (EBNA-1, -2, -3, -4, and -6). To circumvent interference by autoantibodies in the sera, fusion proteins synthesized in Escherichia coli were used as specific antigens. By ELISA the average IgG antibody titers to domains of the five EBNAs, especially the amino-terminal domain of EBNA-2, in sera of patients with Sjögren's syndrome were slightly higher than those in normal sera. The tendency of sera from Sjögren's syndrome patients to have higher reactivities to the EBNA domains was also observed by immunoblotting. By comparison, few heightened serologic responses to EBNAs were observed in sera from patients with rheumatoid arthritis. Further analyses are required to determine if EBV is associated with Sjögren's syndrome in any way.
为了研究爱泼斯坦-巴尔病毒（EBV）感染是否与两种自身免疫性疾病——干燥综合征和类风湿性关节炎相关，对这些疾病患者血清的IgG抗体滴度针对EBV核抗原的五种成分（EBNA-1、-2、-3、-4和-6）进行了评估。为避免血清中自身抗体的干扰，将在大肠杆菌中合成的融合蛋白用作特异性抗原。通过酶联免疫吸附测定（ELISA），干燥综合征患者血清中针对五种EBNA结构域，尤其是EBNA-2氨基末端结构域的平均IgG抗体滴度略高于正常血清。通过免疫印迹法也观察到干燥综合征患者血清对EBNA结构域具有更高反应性的趋势。相比之下，类风湿性关节炎患者血清中未观察到对EBNA的血清学反应增强。需要进一步分析以确定EBV是否以任何方式与干燥综合征相关。
