Panyathep Atita, Punturee Khanittha, Chewonarin Teera
School of Medicine, Mae Fah Luang University, Chiang Rai 57100, Thailand.
Cancer Research Unit of Associated Medical Sciences (AMS-CRU), Faculties of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.
Foods. 2023 Jul 9;12(14):2648. doi: 10.3390/foods12142648.
An inflammatory response, related to colorectal cancer (CRC) progression, is a major subsequent result of bacterial infection following CRC surgery and should be of serious concern. Lipopolysaccharide (LPS), from the bacterial membrane, is a vital mediator of this event through binding with a Toll-like receptor 4 (TLR4) and activating through NF-κB in CRC. To identify a novel inhibitor of LPS-induced colon cancer cells (SW480), green coffee bean extract (GBE) was investigated. Ethyl acetate insoluble fraction (EIF) was mainly collected from GBE and classified as chlorogenic acid (CGA)-rich fractions. EIF and CGA inhibited TLR4 expression in LPS-induced SW480 cells. However, EIF was more dominant than CGA, via inhibition of expression and secretion of several associated mediators in inflammatory responses and CRC metastasis through NF-κB inactivation, which resulted in the abrogation of CRC migration and invasion. Thus, CGA-rich fraction from GBE can be further developed as an alternative treatment, coupled with CRC surgical treatment, to increase therapeutic efficiency and survival rate.
与结直肠癌（CRC）进展相关的炎症反应是CRC手术后细菌感染的主要后续结果，应予以严重关注。来自细菌膜的脂多糖（LPS）是这一事件的重要介质，它通过与Toll样受体4（TLR4）结合并在CRC中通过NF-κB激活来发挥作用。为了鉴定一种新型的LPS诱导的结肠癌细胞（SW480）抑制剂，对绿咖啡豆提取物（GBE）进行了研究。乙酸乙酯不溶部分（EIF）主要从GBE中收集，并归类为富含绿原酸（CGA）的部分。EIF和CGA抑制LPS诱导的SW480细胞中TLR4的表达。然而，EIF比CGA更具优势，它通过NF-κB失活抑制炎症反应和CRC转移中几种相关介质的表达和分泌，从而导致CRC迁移和侵袭的消除。因此，GBE中富含CGA的部分可以进一步开发为一种替代治疗方法，与CRC手术治疗相结合，以提高治疗效果和生存率。
