Liu Zewen, Han Zhaojun
Key Laboratory of Monitoring and Management of Plant Disease and Insects, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China.
Pest Manag Sci. 2006 Mar;62(3):279-82. doi: 10.1002/ps.1169.
Imidacloprid has been used as a key insecticide to control the brown planthopper, Nilaparvata lugens Stål, for several years, but no obvious resistance has been identified in field populations as yet. To evaluate the risk, a field population was collected and selected with imidacloprid in the laboratory. After 37-generation selection a strain with 250-fold resistance had been successfully achieved. Fitness analysis by constructing life tables demonstrated that resistant hoppers had obvious disadvantages in their reproduction. The fitness of highly resistant hoppers had decreased dramatically (0.169 and 0.104) to only one-fifth to one-tenth of that of the susceptible strain. Hence it was concluded that the brown planthopper had the potential to develop high resistance to imidacloprid but that the lower fitness of resistant hoppers could result in a quick recovery of sensitivity when the population did not come into contact with imidacloprid. This means that a reasonable resistance management programme with less imidacloprid use may efficiently delay or even stop resistance development.
吡虫啉已被用作防治褐飞虱（Nilaparvata lugens Stål）的关键杀虫剂数年，但目前尚未在田间种群中发现明显的抗性。为评估风险，采集了一个田间种群并在实验室用吡虫啉进行筛选。经过37代筛选，成功获得了具有250倍抗性的品系。通过构建生命表进行适合度分析表明，抗性褐飞虱在繁殖方面存在明显劣势。高抗性褐飞虱的适合度大幅下降（分别为0.169和0.104），仅为敏感品系的五分之一到十分之一。因此得出结论，褐飞虱有对吡虫啉产生高抗性的潜力，但抗性褐飞虱较低的适合度可能导致种群在不接触吡虫啉时敏感性迅速恢复。这意味着合理的抗性治理方案，减少吡虫啉的使用，可能有效地延缓甚至阻止抗性发展。
