Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.
Hubei Engineering Technology Center for Pest Forewarning and Management, College of Agriculture, Yangtze University, Jingzhou 434025, China.
Toxins (Basel). 2022 Jul 1;14(7):453. doi: 10.3390/toxins14070453.
Afidopyropen, a newly identified chemical, is a derivative of pyripyropene A, which is produced by the filamentous fungus . It is a promising novel pesticide applied against whiteflies in agriculture. In this study, the reversion and selection, cross-resistance patterns, synergistic effects, and fitness costs of afidopyropen resistance were studied in a field-developed resistant population of . Compared to a reference MED-S strain, the field-developed resistant Haidian (HD) population showed 36.5-fold resistance to afidopyropen. Significant reversion of resistance to afidopyropen was found in the HD population when it was kept with no selective pressure of the insecticide. The HD-Afi strain, developed from the HD population with afidopyropen pressure, developed 104.3-fold resistance to afidopyropen and significant cross-resistance to sulfoxaflor. Piperonyl butoxide (PBO) largely inhibited afidopyropen resistance in the HD-Afi strain, which indicates that P450 monooxygenase could be involved in the resistance. Significant fitness costs associated with afidopyropen resistance were observed in HD-Afi. This study indicates that a rotation of afidopyropen with other chemical control agents could be useful for impeding afidopyropen resistance in . In addition, we expanded upon the understanding of resistance to afidopyropen, offering evidence suggesting the importance of devising better strategies for the management of whiteflies.
阿弗迪菌素，一种新鉴定的化学物质，是吡丙醚 A 的衍生物，由丝状真菌产生。它是一种有前途的新型农药，用于农业防治粉虱。本研究以田间选育的抗药性种群为研究对象，研究了阿弗迪菌素抗性的回复与选择、交互抗性模式、增效作用和适合度代价。与参考 MED-S 菌株相比，田间选育的抗药性海淀（HD）种群对阿弗迪菌素表现出 36.5 倍的抗性。在没有杀虫剂选择性压力的情况下，HD 种群对阿弗迪菌素的抗性显著回复。从具有阿弗迪菌素压力的 HD 种群中选育出的 HD-Afi 菌株对阿弗迪菌素表现出 104.3 倍的抗性和对磺酰氟的显著交互抗性。增效醚（PBO）对 HD-Afi 菌株的阿弗迪菌素抗性有很大的抑制作用，表明 P450 单加氧酶可能参与了抗性的产生。在 HD-Afi 中观察到与阿弗迪菌素抗性相关的显著适合度代价。本研究表明，阿弗迪菌素与其他化学防治剂轮用可能有助于阻止在 中阿弗迪菌素抗性的发展。此外，我们扩展了对阿弗迪菌素抗性的认识，为制定更好的粉虱管理策略提供了证据。
