Riter Leah S, Gooding Karen M, Hodge Barry D, Julian Randall K
Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, IN 46285, USA.
Proteomics. 2006 Mar;6(6):1735-40. doi: 10.1002/pmic.200500477.
A critical evaluation of the performance of a 2-D linear ion trap (IT) instrument to two 3-D quadrupole IT instruments with emphasis on identification of rat serum proteins by bottom-up LC-MS/MS is presented. The speed and sensitivity of each of the instruments were investigated, and the effects that each of these have on the bottom-up proteomics identification approach are discussed.
本文对二维线性离子阱（IT）仪器与两台三维四极杆离子阱仪器的性能进行了批判性评估，重点是通过自下而上的液相色谱-串联质谱法（LC-MS/MS）鉴定大鼠血清蛋白。研究了每台仪器的速度和灵敏度，并讨论了它们各自对自下而上蛋白质组学鉴定方法的影响。
