Jakubowska Agata K, Peters Sander A, Ziemnicka Jadwiga, Vlak Just M, van Oers Monique M
Laboratory of Virology, Wageningen University, Binnenhaven 11, 6709 PD Wageningen, The Netherlands.
Department of Biological Control and Quarantine, Institute of Plant Protection, Miczurina 20, Poznan 60-318, Poland.
J Gen Virol. 2006 Mar;87(Pt 3):537-551. doi: 10.1099/vir.0.81461-0.
The genome sequence of a Polish isolate of Agrotis segetum nucleopolyhedrovirus (AgseNPV-A) was determined and analysed. The circular genome is composed of 147,544 bp and has a G+C content of 45.7 mol%. It contains 153 putative, non-overlapping open reading frames (ORFs) encoding predicted proteins of more than 50 aa, together making up 89.8 % of the genome. The remaining 10.2 % of the DNA constitutes non-coding regions and homologous-repeat regions. One hundred and forty-three AgseNPV-A ORFs are homologues of previously reported baculovirus gene sequences. There are ten unique ORFs and they account for 3 % of the genome in total. All 62 lepidopteran baculovirus genes, including the 29 core baculovirus genes, were found in the AgseNPV-A genome. The gene content and gene order of AgseNPV-A are most similar to those of Spodoptera exigua (Se) multiple NPV and their shared homologous genes are 100 % collinear. Three putative enhancin genes were identified in the AgseNPV-A genome. In phylogenetic analysis, the AgseNPV-A enhancins form a cluster separated from enhancins of the Mamestra species NPVs.
测定并分析了波兰黄地老虎核型多角体病毒（AgseNPV - A）分离株的基因组序列。该环状基因组由147,544个碱基对组成，G + C含量为45.7摩尔％。它包含153个推定的、不重叠的开放阅读框（ORF），编码预测的50个以上氨基酸的蛋白质，这些蛋白质总共占基因组的89.8％。其余10.2％的DNA构成非编码区和同源重复区。143个AgseNPV - A的ORF与先前报道的杆状病毒基因序列同源。有10个独特的ORF，它们总共占基因组的3％。在AgseNPV - A基因组中发现了所有62个鳞翅目杆状病毒基因，包括29个核心杆状病毒基因。AgseNPV - A的基因含量和基因顺序与甜菜夜蛾（Se）多粒NPV最为相似，它们共享的同源基因100％共线。在AgseNPV - A基因组中鉴定出三个推定的增强蛋白基因。在系统发育分析中，AgseNPV - A增强蛋白形成一个与甘蓝夜蛾属NPV的增强蛋白分离的簇。
