Emerson Suzanne U, Clemente-Casares Pilar, Moiduddin Nasser, Arankalle Vidya A, Torian Udana, Purcell Robert H
Molecular Hepatitis and Hepatitis Viruses Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 50 South Drive MSC-8009, Bethesda, MD 20892-8009, USA.
Hepatitis Division, National Institute of Virology, Pune, India.
J Gen Virol. 2006 Mar;87(Pt 3):697-704. doi: 10.1099/vir.0.81545-0.
Monolayers of Hep G2/C3A cells were inoculated with genotype 1 Hepatitis E virus (HEV) mixed with either anti-HEV or an appropriate control. After 5 or 6 days, cell monolayers were stained with anti-HEV and infected cells were identified by immunofluorescence microscopy and counted. Anti-HEV from vaccinated or infected rhesus monkeys neutralized the virus, as did mAbs that recognized epitopes on the C terminus of a recombinant vaccine protein. Antibodies were broadly cross-reactive, since convalescent serum from animals infected with any one of the four mammalian genotypes all neutralized the genotype 1 virus.
将基因型1戊型肝炎病毒（HEV）与抗HEV或适当对照混合后接种到Hep G2/C3A细胞单层中。5或6天后，用抗HEV对细胞单层进行染色，通过免疫荧光显微镜鉴定并计数感染细胞。来自接种疫苗或感染的恒河猴的抗HEV中和了病毒，识别重组疫苗蛋白C末端表位的单克隆抗体也有同样效果。抗体具有广泛的交叉反应性，因为感染四种哺乳动物基因型中任何一种的动物的恢复期血清均能中和基因型1病毒。
