Wang Ya-Ying, Lü Dan, Wei Dong-Mei, Lin Wen-Xiong, Tian Hui-Qiao
School of Life Sciences, Xiamen University, Xiamen 361005, China.
Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao. 2006 Feb;32(1):113-22.
The distribution of ATPase was studied using lead precipitation technique during anther development in rice. The ATPase reactive precipitates (ppts) were located mostly in the nucleus of microspore mother cells (MMC) and only a few in the cytoplasm (Plate I-1). Anther wall had differentiated into four layers of cells and a few precipitates were located in the cells except the nucleus of tapetal cells where there were many ATPase reactive precipitates (Plate I-2). After meiosis of MMC, tapetal cells formed many endoplasmic reticula in its cytoplasm but still contained a few ppts. In the cells of epidermis, endothelium and middle layer, the ppts increased evidently in plasma membrane and near cytoplasm than before (Plate I-5). There were a large number of ppts located in the pollen wall during pollen development (Plate I-6), suggesting that ATPase is necessary for the construction of pollen wall. The exine of pollen wall of rice was constructed during microspore development and consisted of sporopollenin which came from tapetal cells. The ppts in exine also came from tapetal cell (Plate II-7). The intine of pollen wall was constructed during the stage of 2-cellular pollen and consisted of cellulose material coming from vegetative cell of pollen. The ATPase and ppts in intine came from vegetative cell of pollen (Plate III-7). Vegetative cell contained more ppts than generative cell during the development of 2-cellular pollen (Plate II-4, 5). The amount of ppts between two sperm cells in a pollen grain was also different (Plate IV-3,4). The physiological functions of ATPase located in different cells and different parts in the cells during anther development of rice were analyzed.
利用铅沉淀技术研究了水稻花药发育过程中ATP酶的分布。ATP酶反应沉淀物（ppt）主要位于小孢子母细胞（MMC）的细胞核中，细胞质中只有少数（图版I - 1）。花药壁已分化为四层细胞，除绒毡层细胞核外，细胞中有少数沉淀物，而绒毡层细胞核中有许多ATP酶反应沉淀物（图版I - 2）。MMC减数分裂后，绒毡层细胞在其细胞质中形成许多内质网，但仍含有少数ppt。在表皮、内皮和中层细胞中，质膜和细胞质附近的ppt明显比以前增加（图版I - 5）。花粉发育过程中，花粉壁中有大量ppt（图版I - 6），表明ATP酶对花粉壁的构建是必需的。水稻花粉壁的外壁在小孢子发育过程中形成，由来自绒毡层细胞的孢粉素组成。外壁中的ppt也来自绒毡层细胞（图版II - 7）。花粉壁的内壁在二细胞花粉阶段形成，由来自花粉营养细胞的纤维素物质组成。内壁中的ATP酶和ppt来自花粉营养细胞（图版III - 7）。在二细胞花粉发育过程中，营养细胞中的ppt比生殖细胞多（图版II - 4、5）。花粉粒中两个精细胞之间的ppt数量也不同（图版IV - 3、4）。分析了水稻花药发育过程中位于不同细胞和细胞不同部位的ATP酶的生理功能。
