Guan He Xin, Tian Hui Qiao, Wang Li, Zhu Ying Guo, Lan Sheng Yin, Xu Zhen Xiu
Agricultural College, Guangxi University, Nanning 530004.
Shi Yan Sheng Wu Xue Bao. 2002 Jun;35(2):109-16.
The development of mature tracheary elements (TEs) of rice (Oryza sativa L. cv. Nongkeng 58s-SD) stamen from big-vacuole to trinucleate pollen stage was studied with lead phosphate--deposition technique. At big-vacuole pollen stage, mature TEs, both in filament and connective, were short of ATPase activity. However, ATPase activity appeared at binucleate pollen stage; in filament, one or two vascular bundle cell (s) next to TE degenerated earlier than the rest; and the plasma membrane of degenerating cell (DC) always exhibited high ATPase activity on condition that the adjoining TE was of fully stretched second wall; when the second wall only turned up far off DC, the plasma membrane of DC still showed high ATPase activity except on borderline between DC and TE; in connective at the same stage, TEs also exhibited high ATPase activity, but the vascular bundle cell(s) adjoining TEs degenerated much later than their counterparts in filament. At trinucleate pollen stage, materials, either with or without ATPase activity, were observed both in filament and connective TEs. The above results indicated that (some of the) materials in mature TEs come from filament DC.
采用焦磷酸铅沉淀技术，研究了水稻（Oryza sativa L. cv. Nongkeng 58s-SD）雄蕊成熟管状分子（TEs）从大液泡花粉期到三核花粉期的发育过程。在大液泡花粉期，花丝和药隔中的成熟TEs均缺乏ATP酶活性。然而，ATP酶活性在二核花粉期出现；在花丝中，紧邻TEs的一或两个维管束细胞比其他细胞更早退化；并且在相邻TEs具有完全伸展的次生壁的情况下，退化细胞（DC）的质膜始终表现出高ATP酶活性；当次生壁仅在远离DC处出现时，DC的质膜除了在DC与TEs的边界处外仍显示高ATP酶活性；在同一时期的药隔中，TEs也表现出高ATP酶活性，但紧邻TEs的维管束细胞比花丝中的对应细胞退化得晚得多。在三核花粉期，在花丝和药隔TEs中均观察到有或无ATP酶活性的物质。上述结果表明，成熟TEs中的（部分）物质来自花丝DC。
