Zhou Zhu Qing, Lan Sheng Yin, Xu Zhen Xiu, Yang Ze Min
College of life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Shi Yan Sheng Wu Xue Bao. 2005 Feb;38(1):7-15.
The ultracytochemical localizations of ATPase activity on amyloplasts and protein bodies were studied by using a lead precipitation technique at middle and late developmental stage of endosperm in rice (Oryza sativa L. cv. Minghui 63). The results showed that the inner and outer plasma membrane of amyloplasts, the web-like passages among starch grains and the amorphous substance surrounding the amyloplasts exhibited high ATPase activity. ATPase activity products were present on the membranes of protein body I and protein body II, and on the vacuoles and vesicle-like structures surrounding protein bodies. Active products of ATPase were also located on the plasma membrane and cell wall of starchy endosperm cells, on the cell wall, plasma membrane, nucleus and plasmodesma of aleurone and subaleurone layer cells. According to the distribution pattern of active products of ATPase, we infer that the web-like passage in amyloplast may be the transporting channel for nutrients flowing into the amyloplast. The ATPase on amyloplasts and protein bodies can supply the power for nutrients passing through the plasma membranes.
利用铅沉淀技术，对水稻（Oryza sativa L. cv. Minghui 63）胚乳发育中后期淀粉体和蛋白体上ATP酶活性进行了超微细胞化学定位研究。结果表明，淀粉体的内外质膜、淀粉粒间的网状通道以及淀粉体周围的无定形物质呈现出较高的ATP酶活性。ATP酶活性产物存在于I型蛋白体和II型蛋白体的膜上，以及蛋白体周围的液泡和囊泡状结构上。ATP酶的活性产物还位于淀粉胚乳细胞的质膜和细胞壁上，糊粉层和亚糊粉层细胞的细胞壁、质膜、细胞核和胞间连丝上。根据ATP酶活性产物的分布模式，我们推测淀粉体内的网状通道可能是营养物质流入淀粉体的运输通道。淀粉体和蛋白体上的ATP酶可为营养物质穿过质膜提供动力。
