Murch Susan J, Peiris Sriyani E, Shi Wendy L, Zobayed S M A, Saxena Praveen K
Department of Chemistry, I. K. Barber School of Arts & Sciences, University of British Columbia, Okanagan, Kelowna, British Columbia, Canada, V1V 1V7.
Plant Cell Rep. 2006 Jun;25(6):522-32. doi: 10.1007/s00299-006-0118-5. Epub 2006 Feb 14.
The production of new varieties and higher quality products from Echinacea spp. requires a greater understanding of the regulation of plant growth and the production of specific phytometabolites. The current studies were designed to generate elite varieties of Echinacea purpurea based on regeneration efficiency and chemical profile. Clonal propagation of seedling-derived regenerants and screening for antioxidant potential and concentrations of caftaric acid, chlorogenic acid, cichoric acid, cynarin, and echinacoside identified 58 unique germplasm lines. Chemical profiles varied significantly among germplasm lines but were consistent within clones of each line. In temporary immersion bioreactors, exogenous application of the auxin indolebutyric acid significantly increased the cichoric acid and caftaric acid concentration in the root tissues. Together, these demonstrate the potential for selective breeding of elite, highly regenerative, chemically superior, clonally propagated varieties from the naturally occurring genetic variability in the seed populations of E. purpurea.
从紫锥菊属植物中培育出新的品种和更高质量的产品，需要更深入地了解植物生长调控以及特定植物代谢产物的生成。当前的研究旨在基于再生效率和化学特征培育紫锥菊的优良品种。对由幼苗衍生的再生植株进行克隆繁殖，并筛选其抗氧化潜力以及咖啡酸、绿原酸、菊苣酸、洋蓟素和紫锥菊苷的浓度，从而确定了58个独特的种质系。种质系之间的化学特征差异显著，但每个系的克隆内特征一致。在临时浸没式生物反应器中，外源施加生长素吲哚丁酸可显著提高根组织中菊苣酸和咖啡酸的浓度。这些研究共同证明，利用紫锥菊种子群体中天然存在的遗传变异，有潜力选择性培育出优良、高再生能力、化学性质优良且可克隆繁殖的品种。
