Jones Maxwell P A, Yi Zhijun, Murch Susan J, Saxena Praveen K
Department of Plant Agriculture, University of Guelph, Guelph, ON, N1G 2W1, Canada.
Plant Cell Rep. 2007 Jan;26(1):13-9. doi: 10.1007/s00299-006-0209-3. Epub 2006 Aug 2.
The goals of this study were to investigate thidiazuron (TDZ)-induced morphogenesis of Echinacea purpurea L. and to assess the possibility of developing a liquid-based protocol for rapid micropropagation. Callus development and root organogenesis were observed on leaf explants cultured on media containing 2,4-dicholorophenoxyacetic acid or dicamba, but no plantlets were regenerated. Addition of TDZ to the culture medium as the sole growth regulator resulted in the production of regenerable callus cultures. The highest rate of regeneration was observed for explants cultured on medium with TDZ at 2.5 microM or higher. Tissue derived from 1.0 microM TDZ treatments was used to initiate liquid cultures. All liquid treatments produced a similar number of regenerants but significantly more healthy plants were obtained from cultures grown in the presence of 0.1 and 1.0 microM TDZ. This TDZ-based micropropagation system is the first liquid, large-scale propagation protocol developed for the mass production of E. purpurea plants.
本研究的目的是研究噻苯隆（TDZ）诱导紫锥菊的形态发生，并评估开发一种基于液体的快速微繁殖方案的可能性。在含有2,4-二氯苯氧乙酸或麦草畏的培养基上培养的叶片外植体上观察到愈伤组织的发育和根器官发生，但未再生出植株。作为唯一的生长调节剂向培养基中添加TDZ导致产生可再生的愈伤组织培养物。在含有2.5 microM或更高浓度TDZ的培养基上培养的外植体观察到最高的再生率。来自1.0 microM TDZ处理的组织用于启动液体培养。所有液体处理产生的再生植株数量相似，但在0.1和1.0 microM TDZ存在下培养的植株获得的健康植株明显更多。这种基于TDZ的微繁殖系统是首个为大规模生产紫锥菊植株而开发的液体、大规模繁殖方案。
