Furuya M, Yoshida M, Hayashi Y, Ohnuma N, Minamino N, Kangawa K, Matsuo H
Suntory Institute for Biomedical Research, Shimamoto, Osaka, Japan.
Biochem Biophys Res Commun. 1991 Jun 28;177(3):927-31. doi: 10.1016/0006-291x(91)90627-j.
C-type natriuretic peptide (CNP) which potently stimulates particulate guanylate cyclase activity in cultured rat vascular smooth muscle cells (VSMC) inhibited serum-induced DNA synthesis of the cells 10-fold more effectively than alpha-human atrial natriuretic peptide (alpha-hANP). The inhibitory effect of CNP was mimicked by 8-bromo-cGMP. The proliferation of VSMC was also suppressed by CNP more potently than alpha-hANP, while the peptide was less active for cGMP augmentation and for vasorelaxation than alpha-hANP in isolated rat aorta. These results suggest that CNP may be a growth regulating factor of VSMC rather than a vasodilator.
C型利钠肽（CNP）能有效刺激培养的大鼠血管平滑肌细胞（VSMC）中的颗粒型鸟苷酸环化酶活性，其抑制细胞血清诱导的DNA合成的效果比α-人心房利钠肽（α-hANP）强10倍。8-溴-cGMP可模拟CNP的抑制作用。与α-hANP相比，CNP对VSMC增殖的抑制作用也更强，而在离体大鼠主动脉中，该肽在增强cGMP和血管舒张方面的活性比α-hANP弱。这些结果表明，CNP可能是VSMC的生长调节因子而非血管舒张剂。
