Murray B W, McClymont R A, Strobeck C
Department of Biology, McMaster University, Hamilton, Ontario, Canada.
J Forensic Sci. 1995 Nov;40(6):943-51.
A survey of mitochondrial D-loop variation in 15 species of ungulates was conducted via amplification by the polymerase chain reaction followed by restriction fragment length polymorphism analysis. This survey included moose (Alces alces), caribou (Rangifer tarandus), mule deer (Odocoileus hemionus hemionus), black-tailed deer (O. h. columbianus), white-tailed deer (O. virginianus), waipiti (Cervus elaphus), pronghorn antelope (Antilocapra americana), bighorn sheep (Ovis canadensis), Stone's sheep (O. dalli), domestic sheep (O. aries), moulflon sheep (O. musimon), mountain goat (Oreamnos americanus), domestic goat (Capra hircus), domestic cattle (Bos taurus), and bison (Bison bison). The results of this preliminary survey indicate that there may be sufficient species specific variation in the D-loop region of the mitochondrial genome of the ungulate species examined here, with the exception of deer (Odocoileus) species, to establish the species origin of the mitochondrial haplotypes of this group. The Odocoileus species are known to hybridize and sharing of mtDNA haplotypes was observed. The chelex DNA extraction technique was successfully used on small blood stains.
通过聚合酶链反应扩增，随后进行限制性片段长度多态性分析，对15种有蹄类动物的线粒体D环变异进行了调查。该调查包括驼鹿（Alces alces）、驯鹿（Rangifer tarandus）、骡鹿（Odocoileus hemionus hemionus）、黑尾鹿（O. h. columbianus）、白尾鹿（O. virginianus）、马鹿（Cervus elaphus）、叉角羚（Antilocapra americana）、大角羊（Ovis canadensis）、斯通氏羊（O. dalli）、家羊（O. aries）、摩弗伦羊（O. musimon）、山羊（Oreamnos americanus）、家山羊（Capra hircus）、家牛（Bos taurus）和野牛（Bison bison）。本次初步调查结果表明，除鹿（Odocoileus）物种外，在此处检测的有蹄类动物物种的线粒体基因组D环区域可能存在足够的物种特异性变异，以确定该群体线粒体单倍型的物种起源。已知Odocoileus物种会杂交，并且观察到线粒体DNA单倍型的共享情况。螯合树脂DNA提取技术成功应用于小血渍。
