Tang Y, Schwarting R, Stein H
Institute of Pathology, Klinikum Steglitz, Freien Universitaet Berlin, Federal Republic of Germany.
Zhonghua Yi Xue Za Zhi. 1991 Feb;71(2):78-80, 8.
Using the monoclonal antibody Ki-67 that detects a cell proliferation-associated human nuclear antigen, incorporations of bromodeoxyuridine (Brud) and 3H-Thymidine, we studied the proliferative capacity of Reed-Sternberg (RS) cells in Hodgkin's cell lines L428, KM-H2, Holden, L540 and L591. The results revealed that in all cell lines, except L591, more than 55% of RS cells were found positive for Ki-67, and over 50% of them had Brud incorporation 5 days after cultivation. The positive score for 3H-Thymidine incorporation was low, and it is assumed that the time taken for 3H-Thymidine in cultivation was short. It shows that at least half of RS cells in these cell lines have proliferation-associated nuclear antigen or can synthesize DNA, and so they are proliferative.
利用可检测细胞增殖相关人类核抗原的单克隆抗体Ki-67、溴脱氧尿苷（Brud）掺入法和3H-胸腺嘧啶核苷掺入法，我们研究了霍奇金细胞系L428、KM-H2、Holden、L540和L591中里德-施特恩伯格（RS）细胞的增殖能力。结果显示，除L591外，在所有细胞系中，超过55%的RS细胞Ki-67呈阳性，且培养5天后超过50%的细胞有Brud掺入。3H-胸腺嘧啶核苷掺入的阳性评分较低，推测培养中3H-胸腺嘧啶核苷的培养时间较短。这表明这些细胞系中至少一半的RS细胞具有增殖相关核抗原或能够合成DNA，因此它们具有增殖能力。
