Hsu P L, Lin Y C, Hsu S M
Department of Pathology, University of Arkansas for Medical Sciences, Little Rock.
Int J Hematol. 1991 Aug;54(4):315-26.
The authors studied the production of macrophage colony-stimulating factor (M-CSF) and the expression of its receptor (c-fms) in two Hodgkin's Reed-Sternberg (H-RS) cell lines, HDLM-1 and KM-H2 and in H-RS cells in tissues. We found that both types of H-RS cell can produce M-CSF, as was confirmed by the presence of M-CSF mRNA and protein in the cells and by the presence of macrophage colony-stimulating activity in conditioned medium. M-CSF was also expressed by H-RS cells in lymph nodes from patients with Hodgkin's disease. In cultures, KM-H2 cells appeared to produce a lesser amount of M-CSF than did HDLM-1 cells, as indicated by weaker staining with anti-M-CSF in the former cells. In KM-H2 cells, most of the M-CSF was located in the cytoplasm, and in HDLM-1 cells, in the Golgi apparatus and/or on the cell membrane. The two types of cultured H-RS cell either did not express c-fms at all, or expressed it only extremely weakly, perhaps because of the loss of dependence on specific growth factors during culture. The production of M-CSF by H-RS cells may contribute to the clinical and pathologic changes seen in patients with Hodgkin's disease, such as the increased abundance of histiocytes in tissues infiltrated by H-RS cells. Alternatively, the expression of both M-CSF and c-fms could confer a growth advantage to some H-RS cells in an autocrine fashion.
作者研究了巨噬细胞集落刺激因子（M-CSF）的产生及其受体（c-fms）在两种霍奇金里德-斯腾伯格（H-RS）细胞系HDLM-1和KM-H2以及组织中的H-RS细胞中的表达。我们发现，两种类型的H-RS细胞均可产生M-CSF，细胞中M-CSF mRNA和蛋白的存在以及条件培养基中巨噬细胞集落刺激活性的存在证实了这一点。霍奇金病患者淋巴结中的H-RS细胞也表达M-CSF。在培养物中，KM-H2细胞产生的M-CSF似乎比HDLM-1细胞少，前者细胞中抗M-CSF染色较弱表明了这一点。在KM-H2细胞中，大部分M-CSF位于细胞质中，而在HDLM-1细胞中，位于高尔基体和/或细胞膜上。两种培养的H-RS细胞要么根本不表达c-fms，要么表达极其微弱，这可能是因为在培养过程中对特定生长因子的依赖性丧失。H-RS细胞产生M-CSF可能导致霍奇金病患者出现临床和病理变化，例如H-RS细胞浸润组织中组织细胞数量增加。或者，M-CSF和c-fms的表达都可以以自分泌方式赋予一些H-RS细胞生长优势。
