Gerdes J, Van Baarlen J, Pileri S, Schwarting R, Van Unnik J A, Stein H
Am J Pathol. 1987 Sep;128(3):390-3.
The growth fraction of tumor cells was studied in 45 cases of Hodgkin's disease by means of a recently developed double immunostaining technique using monoclonal antibody Ki-1, which reacts selectively with Hodgkin and Reed-Sternberg cells in tissues affected by Hodgkin's disease, and antibody Ki-67, which recognizes a cell proliferation-associated nuclear antigen. The medians of the growth fractions of the tumor cells in all histologic subtypes of Hodgkin's disease varied between 78% and 83%. In none of the cases investigated did we find a growth fraction below 50%. Furthermore, mononucleated Hodgkin cells as well as multi-nucleated Reed-Sternberg cells showed a similar Ki-67 labeling index, indicating that both tumor cell types belong to the proliferating pool of this malignancy.
采用一种最近开发的双重免疫染色技术，使用单克隆抗体Ki-1和Ki-67，对45例霍奇金病患者的肿瘤细胞生长分数进行了研究。单克隆抗体Ki-1可与霍奇金病受累组织中的霍奇金和里德-斯腾伯格细胞发生选择性反应，抗体Ki-67可识别一种与细胞增殖相关的核抗原。霍奇金病所有组织学亚型中肿瘤细胞生长分数的中位数在78%至83%之间。在所有研究病例中，均未发现生长分数低于50%的情况。此外，单核霍奇金细胞和多核里德-斯腾伯格细胞显示出相似的Ki-67标记指数，表明这两种肿瘤细胞类型均属于该恶性肿瘤的增殖池。
