Vidal N, Mulanga C, Bazepeo S Edidi, Lepira F, Delaporte E, Peeters M
UMR145, Laboratoire Retrovirus, IRD, BP 64501, 34394 Montpellier Cedex 1, France.
AIDS Res Hum Retroviruses. 2006 Feb;22(2):182-7. doi: 10.1089/aid.2006.22.182.
Phylogenetic analysis on partial env sequences of HIV-1-positive samples obtained from sentinel population groups in HIV serosurveillance studies in 1997 and 2002 in the Democratic Republic of Congo (DRC) revealed a high genetic diversity in the number of cocirculating subtypes/CRFs as well as intrasubtype diversity. A cluster of three strains that formed a distinct and well-separated group within the subtype A radiation was observed. To determine a more precise structure of these viruses, the full-length genomes were sequenced. Phylogenetic tree and bootscan analysis showed that all three newly characterized viruses formed a new nonrecombinant lineage that was more closely related to subtype A. The SUDI (Subtype Distance) program showed that the distances of the newly derived HIV-1 sequences to subtype A references fell in the range of distances previously characterized for subsubtypes. According to current nomenclature rules, we have thus designated this new lineage as subsubtype A4. Analysis of viral sequences from other African countries suggests that the A4 strains seem restricted to DRC where they already circulated at the onset of the HIV/AIDS epidemic.
对1997年和2002年在刚果民主共和国（DRC）进行的HIV血清学监测研究中从哨点人群组获得的HIV-1阳性样本的部分env序列进行系统发育分析，结果显示，同时流行的亚型/CRF数量以及亚型内多样性存在高度遗传多样性。观察到一组三个毒株在A亚型辐射范围内形成了一个独特且分隔良好的群体。为了确定这些病毒更精确的结构，对全长基因组进行了测序。系统发育树和bootscan分析表明，所有三种新鉴定的病毒形成了一个新的非重组谱系，与A亚型关系更密切。SUDI（亚型距离）程序显示，新获得的HIV-1序列与A亚型参考序列的距离落在先前为亚亚型所确定的距离范围内。根据当前的命名规则，我们因此将这个新谱系指定为A4亚亚型。对来自其他非洲国家的病毒序列分析表明，A4毒株似乎仅限于DRC，在HIV/AIDS疫情开始时它们就在那里传播。
