IBB - Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus de Gualtar, Braga, Portugal.
Lett Appl Microbiol. 2010 Apr;50(4):419-24. doi: 10.1111/j.1472-765X.2010.02818.x. Epub 2010 Feb 5.
The aim of this study was to determine the antimicrobial and antiadhesive properties of a biosurfactant isolated from Lactobacillus paracasei ssp. paracasei A20 against several micro-organisms, including Gram-positive and Gram-negative bacteria, yeasts and filamentous fungi.
Antimicrobial and antiadhesive activities were determined using the microdilution method in 96-well culture plates. The biosurfactant showed antimicrobial activity against all the micro-organisms assayed, and for twelve of the eighteen micro-organisms (including the pathogenic Candida albicans, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis and Streptococcus agalactiae), the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were achieved for biosurfactant concentrations between 25 and 50 mg ml(-1). Furthermore, the biosurfactant showed antiadhesive activity against most of the micro-organisms evaluated.
As far as we know, this is the first compilation of data on antimicrobial and antiadhesive activities of biosurfactants obtained from lactobacilli against such a broad group of micro-organisms. Although the antiadhesive activity of biosurfactants isolated from lactic acid bacteria has been widely reported, their antimicrobial activity is quite unusual and has been described only in a few strains.
The results obtained in this study regarding the antimicrobial and antiadhesive properties of this biosurfactant opens future prospects for its use against micro-organisms responsible for diseases and infections in the urinary, vaginal and gastrointestinal tracts, as well as in the skin, making it a suitable alternative to conventional antibiotics.
本研究旨在确定从副干酪乳杆菌亚种副干酪乳杆菌 A20 中分离出的生物表面活性剂对包括革兰氏阳性和革兰氏阴性细菌、酵母和丝状真菌在内的几种微生物的抗菌和抗粘附特性。
使用 96 孔培养板中的微量稀释法测定抗菌和抗粘附活性。生物表面活性剂对所有检测的微生物均表现出抗菌活性，对于 18 种微生物中的 12 种（包括致病性白色念珠菌、大肠杆菌、金黄色葡萄球菌、表皮葡萄球菌和无乳链球菌），在 25 至 50mg/ml 的生物表面活性剂浓度下实现了最小抑菌浓度 (MIC) 和最小杀菌浓度 (MBC)。此外，生物表面活性剂对大多数评估的微生物表现出抗粘附活性。
据我们所知，这是首次对来自乳杆菌的生物表面活性剂对如此广泛的微生物群的抗菌和抗粘附活性进行数据汇编。尽管已经广泛报道了从乳酸菌中分离出的生物表面活性剂的抗粘附活性，但它们的抗菌活性是相当不寻常的，并且仅在少数几个菌株中有所描述。
本研究中关于该生物表面活性剂的抗菌和抗粘附特性的结果为其在治疗泌尿系统、阴道和胃肠道以及皮肤疾病和感染中对抗微生物的用途开辟了未来的前景，使其成为传统抗生素的合适替代品。
