Velraeds M M, van de Belt-Gritter B, van der Mei H C, Reid G, Busscher H J
Laboratory for Materia Technica, University of Groningen, The Netherlands.
J Med Microbiol. 1998 Dec;47(12):1081-5. doi: 10.1099/00222615-47-12-1081.
The ability of the Lactobacillus acidophilus RC14 biosurfactant 'surlactin' to inhibit the initial adhesion of various uropathogenic bacteria and two yeast strains to silicone rubber was investigated in a parallel-plate flow chamber in filter-sterilised pooled human urine. A parallel-plate flow chamber with a silicone rubber bottom plate was filled with a 1.0 mg/ml biosurfactant solution for adsorption overnight (18 h). Subsequently, the adhesion of the bacterial or yeast cells from a urine suspension under low flow (shear rate 15 s(-1)) was followed in situ by automated image analysis. Control tests were with untreated silicone rubber. Initial deposition rates and numbers of adhering cells after 4 h of flow were determined. Surlactin layers caused a marked inhibition of the initial deposition rates and adhesion numbers after 4 h for the majority of the bacteria (11 of 15 strains tested) and this inhibition was particularly effective against Enterococcus faecalis, Escherichia coli and Staphylococcus epidermidis. Although the initial deposition rates of the two Candida albicans strains were reduced by c. 50% in comparison with the controls, the numbers of yeast cells adhering after 4 h were similar.
在过滤除菌的混合人尿中，利用平行平板流动腔研究嗜酸乳杆菌RC14生物表面活性剂“表面活性素”抑制各种尿路致病性细菌及两种酵母菌株对硅橡胶初始黏附的能力。将底部为硅橡胶的平行平板流动腔充满1.0 mg/ml生物表面活性剂溶液，吸附过夜（18小时）。随后，通过自动图像分析原位跟踪低流量（剪切速率15 s(-1)）下尿液悬浮液中细菌或酵母细胞的黏附情况。对照试验采用未处理的硅橡胶。测定了初始沉积速率以及流动4小时后黏附细胞的数量。表面活性素层对大多数细菌（15株受试菌株中的11株）的初始沉积速率及4小时后的黏附数量均产生显著抑制作用，且这种抑制作用对粪肠球菌、大肠杆菌和表皮葡萄球菌尤为有效。尽管两种白色念珠菌菌株的初始沉积速率相较于对照降低了约50%，但4小时后黏附的酵母细胞数量相似。
