Lema Pablo Patricio, Girard Christiane, Vachon Pascal
Department of Veterinary Biomedicine and Microbiology and Pathology, Faculty of Veterinary Medicine, University of Montreal, CP 5000, Saint-Hyacinthe, Quebec.
Can J Vet Res. 2005 Oct;69(4):253-9.
Methylprednisolone (MP) was evaluated for the treatment of intracerebral hemorrhage in a Sprague-Dawley rat model of cerebral hematoma induced by subcortical injection of collagenase. At 1 and 24 h after the injection, MP was administered intraperitoneally (IP) at a concentration of 10, 35, or 100 mg/kg. Control groups received saline IP at 1 and 24 h after the intracerebral injection of collagenase (positive controls) or saline (negative controls). Motor behaviour 24 h before and 24 h and 48 h after the intracerebral injection was evaluated by means of a neurologic exam and a rotarod treadmill test. The animals were euthanized at 48 h; brain water content was determined in half of the rats, and histopathological studies were done in the other half. Compared with the positive controls, the animals with collagenase-induced hematoma performed significantly better on the neurologic exam after treatment with 100 mg/kg of MP and on the rotarod test after treatment with 35 or 100 mg/kg of MP. The hematoma volume was significantly smaller (P < 0.002) after all doses of MP; however, the smallest volume was seen with 100 mg/kg. There were significantly fewer neutrophils (P < 0.01) within the hematoma in the MP-treated animals (maximum reduction with 100 mg/kg) than in the positive controls, but the numbers of reactive astrocytes did not differ significantly between the treatment groups. The number of necrotic neurons in the penumbra did not differ between the treatment groups; however, there were significantly fewer (P < 0.005) in the cerebral cortex in the group treated with 100 mg/kg of MP compared with the positive controls. These results suggest that high doses of MP administered shortly after occurrence of a cerebral hematoma are beneficial for the treatment of intracerebral hemorrhage.
在通过皮下注射胶原酶诱导脑血肿的Sprague-Dawley大鼠模型中，对甲基强的松龙（MP）治疗脑出血进行了评估。在注射后1小时和24小时，以10、35或100mg/kg的浓度腹腔注射（IP）MP。对照组在脑内注射胶原酶后1小时和24小时接受腹腔注射生理盐水（阳性对照）或生理盐水（阴性对照）。通过神经学检查和转棒式跑步机试验评估脑内注射前24小时以及注射后24小时和48小时的运动行为。在48小时时对动物实施安乐死；测定一半大鼠的脑含水量，另一半进行组织病理学研究。与阳性对照相比，用100mg/kg MP治疗后，胶原酶诱导血肿的动物在神经学检查中表现明显更好，用35或100mg/kg MP治疗后在转棒试验中表现明显更好。所有剂量的MP治疗后血肿体积均显著减小（P<0.002）；然而，100mg/kg时血肿体积最小。MP治疗组血肿内的中性粒细胞明显少于阳性对照（P<0.01）（100mg/kg时减少最多），但各治疗组间反应性星形胶质细胞数量无显著差异。各治疗组半暗带内坏死神经元数量无差异；然而，与阳性对照相比，100mg/kg MP治疗组大脑皮质中的坏死神经元明显更少（P<0.005）。这些结果表明，脑血肿发生后不久给予高剂量MP对脑出血治疗有益。
