Ferland Catherine, Veilleux-Lemieux D, Vachon Pascal
Department of Vetirenary Biomedicine, University of Montreal, St-Hyacinthe, Canada.
J Am Assoc Lab Anim Sci. 2007 May;46(3):13-6.
We evaluated the effects of buprenorphine (0.05 mg/kg intraperitoneally) after collagenase-induced intracerebral hemorrhage in Sprague-Dawley rats. Methods of evaluation included serum biochemistry, behavioral tests (neurologic exam and rotarod treadmill), and histopathology. Serum biochemistry parameters showed no change after surgery in controls and buprenorphine-treated animals. At 48 h after collagenase injections, the performance of treated rats on the rotarod treadmill test was not significantly different from that of untreated rats, but the neurologic exams of treated rats showed significantly improved performance. Although the volume of the hematoma was reduced with buprenorphine, the number of necrotic neurons in the penumbra was significantly increased. These data indicate that administration of buprenorphine led to neurologic and histopathologic differences in a rat model of intracerebral hemorrhage, and data from such studies should be interpreted carefully if an opioid analgesic is used to minimize pain.
我们评估了在胶原酶诱导的脑出血Sprague-Dawley大鼠模型中，腹腔注射丁丙诺啡（0.05毫克/千克）的效果。评估方法包括血清生化、行为测试（神经学检查和转棒式跑步机试验）以及组织病理学。血清生化参数在对照组和丁丙诺啡治疗组动物术后均无变化。在注射胶原酶48小时后，接受治疗的大鼠在转棒式跑步机试验中的表现与未治疗大鼠无显著差异，但接受治疗大鼠的神经学检查显示表现有显著改善。虽然丁丙诺啡使血肿体积减小，但半暗带中坏死神经元的数量显著增加。这些数据表明，在脑出血大鼠模型中，丁丙诺啡给药导致了神经学和组织病理学差异，并且如果使用阿片类镇痛药来减轻疼痛，此类研究的数据应谨慎解读。
