Del Bigio M R, Yan H J, Buist R, Peeling J
Department of Pathology, University of Manitoba and Health Sciences Centre, Winnipeg, Canada.
Stroke. 1996 Dec;27(12):2312-9; discussion 2319-20. doi: 10.1161/01.str.27.12.2312.
Intracerebral hemorrhage is associated with a considerable proportion of strokes and head injuries. The mechanism of brain cell injury associated with hemorrhage may be different from that due to pure ischemia. Therefore, it is essential that models of intracerebral hemorrhage be developed and well characterized. The purpose of this study was to obtain high-field MR images of rat brain at progressive times after induction of intracerebral hemorrhage and to correlate the images with behavior and histological evolution.
Intracerebral hemorrhage was induced in rats by injection of bacterial collagenase and heparin into the caudate nucleus. Histopathological changes and corresponding MR images were studied from 30 minutes to 3 weeks after injection. Behavioral changes were also followed for 3 weeks.
Histological correlation showed that MR is capable of resolving the accumulation and degeneration of the hematoma, a centripetal wave of neutrophils infiltrating from the surrounding tissue beginning at 12 hours, and centripetal invasion of macrophages beginning at 48 hours. Widespread white matter edema was clearly evident on MR images for 1 week after the hemorrhage. Medium-sized striatal neurons were lost in the tissue surrounding the hematoma. Behavioral improvement was rapid during resolution of the edema but incomplete at 3 weeks.
MR images correlate very well with histological changes in this experimental model of intracerebral hemorrhage and can therefore be used to follow changes due to drug treatments in vivo. The intense neutrophilic response to this lesion may contribute to neuronal injury at the periphery of the hematoma.
脑出血与相当一部分的中风和头部损伤相关。与出血相关的脑细胞损伤机制可能不同于单纯缺血所致的损伤机制。因此，建立并充分表征脑出血模型至关重要。本研究的目的是在诱导脑出血后的不同时间点获取大鼠脑的高场磁共振图像，并将这些图像与行为和组织学演变相关联。
通过向尾状核注射细菌胶原酶和肝素诱导大鼠脑出血。在注射后30分钟至3周内研究组织病理学变化及相应的磁共振图像。行为变化也追踪3周。
组织学相关性显示，磁共振成像能够分辨血肿的积聚和退变，中性粒细胞从12小时开始从周围组织向血肿呈向心性浸润，巨噬细胞从48小时开始向心性侵入。出血后1周，磁共振图像上广泛的白质水肿清晰可见。血肿周围组织中的中等大小纹状体神经元丢失。在水肿消退过程中行为改善迅速，但在3周时仍不完全。
在这个脑出血实验模型中，磁共振图像与组织学变化相关性非常好，因此可用于追踪体内药物治疗引起的变化。对该病变的强烈中性粒细胞反应可能导致血肿周边的神经元损伤。
