Després G, Hafidi A, Romand R
Laboratoire de Neurobiologie, Université Blaise Pascal, Aubière, France.
Hear Res. 1991 Mar;52(1):157-65. doi: 10.1016/0378-5955(91)90195-f.
Nerve growth factor receptor (NGF-R) localization was studied immunohistochemically in the cochlea and in the brainstem of the perinatal rat, using a specific monoclonal antibody directed against the rat NGF-R. In the cochlea, NGF-R immunoreactivity is positive during the whole perinatal period studied, and is located at the hair cell level, in fibers that reach the organ of Corti, in the intraganglionic spiral bundle and in some small bundles of fibers in the auditory nerve. In the brainstem, NGF-R is detected in auditory structures such as the ventral cochlear nucleus, the superior olivary complex, the nuclei of the trapezoid body and the trapezoid body. Many auditory structures labelled by the NGF-R antibody are implicated in the efferent cochlear innervation. These results suggest that NGF could be implicated in interactions between auditory receptors and efferent innervation of the developing cochlea. This coincides with findings on the immunohistochemical localization of NGF-like protein in the organ of Corti of the developing rat. Moreover, these observations could be related to an early prenatal development of auditory efferent innervation.
利用针对大鼠神经生长因子受体（NGF-R）的特异性单克隆抗体，对围产期大鼠的耳蜗和脑干进行免疫组织化学研究，以确定NGF-R的定位。在耳蜗中，在所研究的整个围产期内，NGF-R免疫反应呈阳性，位于毛细胞水平、到达柯蒂氏器的纤维中、螺旋神经节内束以及听神经中的一些小纤维束中。在脑干中，在诸如蜗腹侧核、上橄榄复合体、斜方体核和斜方体等听觉结构中检测到NGF-R。许多被NGF-R抗体标记的听觉结构与耳蜗传出神经支配有关。这些结果表明，NGF可能参与发育中的耳蜗听觉感受器与传出神经支配之间的相互作用。这与在发育中大鼠的柯蒂氏器中NGF样蛋白的免疫组织化学定位结果一致。此外，这些观察结果可能与听觉传出神经支配的早期产前发育有关。
