Zaheer Atif, Murshed Monzur, De Grand Alec M, Morgan Timothy G, Karsenty Gerard, Frangioni John V
Department of Radiology, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA.
Arterioscler Thromb Vasc Biol. 2006 May;26(5):1132-6. doi: 10.1161/01.ATV.0000210016.89991.2a. Epub 2006 Feb 16.
To detect the hydroxyapatite component of vascular calcification in vivo so that the process of calcium deposition can be studied in transgenic model systems.
We have previously developed a near-infrared fluorescent bisphosphonate derivative that binds with high affinity and specificity to hydroxyapatite, and an intraoperative near-infrared fluorescence imaging system for small animals. Using these tools, and a transgenic mouse strain with homozygous deletion of the matrix GLA protein (Mgp(-/-)), we demonstrate that the hydroxyapatite component of vascular calcification can be detected in vivo with high sensitivity, specificity, and resolution.
The hydroxyapatite component of vascular calcification can be detected optically, in real-time, without sacrifice of the animal. It is now possible to study the earliest events associated with vascular mineralization, at the cell and organ level, and to monitor the process in living animals.
检测体内血管钙化的羟基磷灰石成分，以便在转基因模型系统中研究钙沉积过程。
我们先前已开发出一种与羟基磷灰石具有高亲和力和特异性结合的近红外荧光双膦酸盐衍生物，以及一种用于小动物的术中近红外荧光成像系统。利用这些工具，以及基质GLA蛋白纯合缺失的转基因小鼠品系（Mgp(-/-)），我们证明血管钙化的羟基磷灰石成分能够在体内以高灵敏度、特异性和分辨率被检测到。
血管钙化的羟基磷灰石成分能够在不牺牲动物的情况下进行实时光学检测。现在有可能在细胞和器官水平研究与血管矿化相关的最早事件，并在活体动物中监测这一过程。
