Nakayama Akira, del Monte Federica, Hajjar Roger J, Frangioni John V
Beth Israel Deaconess Medical Center, HIM-1023, 330 Brookline Avenue, Boston, MA 02215, USA.
Mol Imaging. 2002 Oct;1(4):365-77. doi: 10.1162/15353500200221333.
Cardiac revascularization is presently performed without real-time visual assessment of myocardial blood flow or perfusion. Moreover, gene therapy of the heart cannot, at present, be directed to specific territories at risk for myocardial infarction. We have developed a surgical imaging system that exploits the low autofluorescence, deep tissue penetration, low tissue scatter, and invisibility of near-infrared (NIR) fluorescent light. By completely isolating visible and NIR light paths, one is able to visualize, simultaneously, the anatomy and/or function of the heart, or any desired tissue. In rat model systems, we demonstrate that the heptamethine indocyanine-type NIR fluorophores IR-786 and the carboxylic acid form of IRDye78 can be injected intravenously in the living animal to provide real-time visual assessment of myocardial blood flow or perfusion intraoperatively. This imaging system may prove useful for the refinement of revascularization techniques, and for the administration of cardiac gene therapy.
目前，心脏血运重建手术在没有对心肌血流或灌注进行实时视觉评估的情况下进行。此外，目前心脏基因治疗无法针对有心肌梗死风险的特定区域。我们开发了一种外科成像系统，该系统利用了近红外（NIR）荧光的低自发荧光、深层组织穿透性、低组织散射以及不可见性。通过完全隔离可见光路和近红外光路，能够同时可视化心脏或任何所需组织的解剖结构和/或功能。在大鼠模型系统中，我们证明七甲川吲哚菁型近红外荧光团IR - 786和IRDye78的羧酸形式可以在活体动物中静脉注射，以在术中对心肌血流或灌注进行实时视觉评估。这种成像系统可能被证明对改进血运重建技术以及心脏基因治疗的实施有用。
