人2号染色体（HSA2）马同源物的1.3兆碱基区间图谱。

A 1.3-Mb interval map of equine homologs of HSA2.

作者信息

Wagner M L, Raudsepp T, Goh G, Agarwala R, Schaffer A A, Dranchak P K, Brinkmeyer-Langford C, Skow L C, Chowdhary B P, Mickelson J R

机构信息

Department of Veterinary Biosciences, College of Veterinary Medicine, University of Minnesota, St Paul, MN 55108, USA.

出版信息

Cytogenet Genome Res. 2006;112(3-4):227-34. doi: 10.1159/000089875.

DOI:10.1159/000089875

PMID:16484777

Abstract

A comparative approach that utilizes information from more densely mapped or sequenced genomes is a proven and efficient means to increase our knowledge of the structure of the horse genome. Human chromosome 2 (HSA2), the second largest human chromosome, comprising 243 Mb, and containing 1246 known genes, corresponds to all or parts of three equine chromosomes. This report describes the assignment of 140 new markers (78 genes and 62 microsatellites) to the equine radiation hybrid (RH) map, and the anchoring of 24 of these markers to horse chromosomes by FISH. The updated equine RH maps for ECA6p, ECA15, and ECA18 resulting from this work have one, two, and three RH linkage groups, respectively, per chromosome/chromosome-arm. These maps have a three-fold increase in the number of mapped markers compared to previous maps of these chromosomes, and an increase in the average marker density to one marker per 1.3 Mb. Comparative maps of ECA6p, ECA15, and ECA18 with human, chimpanzee, dog, mouse, rat, and chicken genomes reveal blocks of conserved synteny across mammals and vertebrates.

摘要

利用来自更密集图谱或测序基因组的信息的比较方法，是增加我们对马基因组结构认识的一种经证实且有效的手段。人类2号染色体（HSA2）是人类第二大染色体，包含243兆碱基对，有1246个已知基因，它对应于三条马染色体的全部或部分。本报告描述了将140个新标记（78个基因和62个微卫星）定位到马辐射杂种（RH）图谱上，并通过荧光原位杂交（FISH）将其中24个标记锚定到马染色体上。这项工作产生的更新后的ECA6p、ECA15和ECA18的马RH图谱，每条染色体/染色体臂分别有一个、两个和三个RH连锁群。与这些染色体以前的图谱相比，这些图谱上定位的标记数量增加了两倍，平均标记密度增加到每1.3兆碱基对一个标记。ECA6p、ECA15和ECA18与人类、黑猩猩、狗、小鼠、大鼠和鸡基因组的比较图谱揭示了跨哺乳动物和脊椎动物的保守同线性区域。