酿酒酵母DNA聚合酶δ亚基中的3'至5'核酸外切酶活性是精确复制所必需的。
The 3' to 5' exonuclease activity located in the DNA polymerase delta subunit of Saccharomyces cerevisiae is required for accurate replication.
作者信息
Simon M, Giot L, Faye G
机构信息
Institut Curie-Biologie, Centre Universitaire, Orsay, France.
出版信息
EMBO J. 1991 Aug;10(8):2165-70. doi: 10.1002/j.1460-2075.1991.tb07751.x.
Abstract
In Saccharomyces cerevisiae, DNA polymerase delta (POLIII), the product of the CDC2 (POL3) gene, possesses, in its N-terminal half, the well conserved 3-domain 3' to 5' exonuclease site. Strains selectively mutagenized in this site display a mutator phenotype detected as a drastically increased spontaneous forward mutation rate to canavanine resistance or as an elevated reversion rate to lysine prototrophy. Assays on a partially purified extract of the mutant giving the largest mutator effect indicate that the 3' to 5' exonuclease activity is reduced below the detection limit whereas the DNA polymerizing activity has wild-type level. Therefore, our results provide experimental support for the hypothesis that the exonucleolytic proofreading activity associated with DNA polymerase delta resides on the DNA polymerase delta subunit and enhances the fidelity of DNA replication in yeast.
摘要
在酿酒酵母中，DNA聚合酶δ（POLIII）是CDC2（POL3）基因的产物，在其N端的一半区域具有保守性良好的3结构域3'至5'核酸外切酶位点。在此位点经选择性诱变的菌株表现出突变体表型，表现为对刀豆氨酸抗性的自发正向突变率大幅增加，或对赖氨酸原养型的回复率升高。对产生最大诱变效应的突变体部分纯化提取物的检测表明，3'至5'核酸外切酶活性降低到检测限以下，而DNA聚合活性具有野生型水平。因此，我们的结果为以下假设提供了实验支持：与DNA聚合酶δ相关的核酸外切校正活性存在于DNA聚合酶δ亚基上，并提高了酵母中DNA复制的保真度。
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本文引用的文献
1
Different Rates of Spontaneous Mutation during Mitosis and Meiosis in Yeast.酵母有丝分裂和减数分裂过程中自发突变的不同速率。
Genetics. 1962 Aug;47(8):1097-108. doi: 10.1093/genetics/47.8.1097.
2
Butylphenyl dGTP: a selective and potent inhibitor of mammalian DNA polymerase alpha.丁基苯基dGTP：一种对哺乳动物DNA聚合酶α具有选择性和强效的抑制剂。
Nucleic Acids Res. 1984 Apr 25;12(8):3695-706. doi: 10.1093/nar/12.8.3695.
3
A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance.酵母中缺乏乳清苷-5'-磷酸脱羧酶活性的突变体的正向选择：5-氟乳清酸抗性。
Mol Gen Genet. 1984;197(2):345-6. doi: 10.1007/BF00330984.
4
Elementary steps in the DNA polymerase I reaction pathway.DNA聚合酶I反应途径中的基本步骤。
Biochemistry. 1983 Jul 19;22(15):3537-46. doi: 10.1021/bi00284a001.
5
Transformation of intact yeast cells treated with alkali cations.经碱金属阳离子处理的完整酵母细胞的转化
J Bacteriol. 1983 Jan;153(1):163-8. doi: 10.1128/jb.153.1.163-168.1983.
6
DNA sequence and expression of the B95-8 Epstein-Barr virus genome.B95-8型爱泼斯坦-巴尔病毒基因组的DNA序列与表达
Nature. 1984;310(5974):207-11. doi: 10.1038/310207a0.
7
Mutations affecting the repressibility of arginine biosynthetic enzymes in Saccharomyces cerevisiae.影响酿酒酵母中精氨酸生物合成酶可阻遏性的突变
Eur J Biochem. 1970 Jan;12(1):31-9. doi: 10.1111/j.1432-1033.1970.tb00817.x.
8
Inheritance of spontaneous mutability in yeast.酵母中自发突变性的遗传
Genetics. 1971 Sep;69(1):17-27. doi: 10.1093/genetics/69.1.17.
9
A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector.基于含着丝粒穿梭载体的酿酒酵母基因组质粒文库。
Gene. 1987;60(2-3):237-43. doi: 10.1016/0378-1119(87)90232-0.
10
Rapid and efficient site-specific mutagenesis without phenotypic selection.无需表型选择的快速高效位点特异性诱变。
Methods Enzymol. 1987;154:367-82. doi: 10.1016/0076-6879(87)54085-x.
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