酿酒酵母DNA聚合酶δ亚基中的3'至5'核酸外切酶活性是精确复制所必需的。
The 3' to 5' exonuclease activity located in the DNA polymerase delta subunit of Saccharomyces cerevisiae is required for accurate replication.
作者信息
Simon M, Giot L, Faye G
机构信息
Institut Curie-Biologie, Centre Universitaire, Orsay, France.
出版信息
EMBO J. 1991 Aug;10(8):2165-70. doi: 10.1002/j.1460-2075.1991.tb07751.x.
Abstract
In Saccharomyces cerevisiae, DNA polymerase delta (POLIII), the product of the CDC2 (POL3) gene, possesses, in its N-terminal half, the well conserved 3-domain 3' to 5' exonuclease site. Strains selectively mutagenized in this site display a mutator phenotype detected as a drastically increased spontaneous forward mutation rate to canavanine resistance or as an elevated reversion rate to lysine prototrophy. Assays on a partially purified extract of the mutant giving the largest mutator effect indicate that the 3' to 5' exonuclease activity is reduced below the detection limit whereas the DNA polymerizing activity has wild-type level. Therefore, our results provide experimental support for the hypothesis that the exonucleolytic proofreading activity associated with DNA polymerase delta resides on the DNA polymerase delta subunit and enhances the fidelity of DNA replication in yeast.
摘要
在酿酒酵母中，DNA聚合酶δ（POLIII）是CDC2（POL3）基因的产物，在其N端的一半区域具有保守性良好的3结构域3'至5'核酸外切酶位点。在此位点经选择性诱变的菌株表现出突变体表型，表现为对刀豆氨酸抗性的自发正向突变率大幅增加，或对赖氨酸原养型的回复率升高。对产生最大诱变效应的突变体部分纯化提取物的检测表明，3'至5'核酸外切酶活性降低到检测限以下，而DNA聚合活性具有野生型水平。因此，我们的结果为以下假设提供了实验支持：与DNA聚合酶δ相关的核酸外切校正活性存在于DNA聚合酶δ亚基上，并提高了酵母中DNA复制的保真度。
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