通过荧光模式下的色谱扫描进行人乳头瘤病毒DNA量的密度测定。
Densitometric determination of human papillomavirus DNA quantities by chromato-scanning in the fluorescence mode.
作者信息
Chinami M, Yuge K, Hachisuka H, Tanikawa E, Goto M, Ohtsu Y, Sasai Y, Shingu M
机构信息
Department of Virology, Kurume University School of Medicine, Japan.
出版信息
J Virol Methods. 1991 Apr;32(1):101-8. doi: 10.1016/0166-0934(91)90189-7.
The quantitation of human papillomavirus DNA isolated from warts by chromato-scanning (fluorescence mode) photographs of ethidium bromide-stained agarose gels is described. Excitation at 200 nm (with a cutoff filter at 400 nm) generates fluorescence from the white portion of the printing paper. The fluorescent intensity correlated with the quantities of DNA in the band of interest. The amounts of DNA were determined using calibration curves of approximately the same size as lambda phage DNA fragments. This general method of quantification is applicable to photographs of other types of polynucleotides capable of being separated and stained in a gel medium.
本文描述了通过对溴化乙锭染色的琼脂糖凝胶进行色谱扫描（荧光模式）照片，对从疣中分离出的人乳头瘤病毒DNA进行定量分析的方法。在200nm波长处激发（使用400nm截止滤光片）可从打印纸的白色部分产生荧光。荧光强度与感兴趣条带中的DNA量相关。使用与λ噬菌体DNA片段大小大致相同的校准曲线来确定DNA的量。这种通用的定量方法适用于其他能够在凝胶介质中分离和染色的多核苷酸的照片。
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