Stewart C Neal
University of Tennessee, Department of Plant Sciences, Knoxville, TN 37996, USA.
Trends Biotechnol. 2006 Apr;24(4):155-62. doi: 10.1016/j.tibtech.2006.02.002. Epub 2006 Feb 20.
Once a biological novelty known for their role in bioluminescence, fluorescent proteins (FPs) from marine invertebrates have revolutionized the life sciences. Organisms from all kingdoms have been transformed with the Aequorea victoria green fluorescent protein (GFP), and biotechnology has been advanced by the use of FPs. This article reviews the current uses of FPs in whole transgenic organisms and genomics and looks beyond GFP to the complete color palette and spectral properties afforded by FPs from other marine organisms. Coupled with electronic devices for visualizing and quantifying FPs, recently cloned FP genes might be useful for the ecological monitoring of transgenic organisms in the environment. Therefore, this review also addresses the in vivo labeling of organisms with an emphasis on plants.
荧光蛋白（FPs）最初是因其在生物发光中的作用而闻名的一种生物学新奇事物，来自海洋无脊椎动物的荧光蛋白彻底改变了生命科学。所有生物界的生物都已被维多利亚多管水母绿色荧光蛋白（GFP）转化，并且荧光蛋白的使用推动了生物技术的发展。本文回顾了荧光蛋白在整个转基因生物和基因组学中的当前用途，并超越了绿色荧光蛋白，探讨了其他海洋生物的荧光蛋白所提供的完整调色板和光谱特性。结合用于可视化和量化荧光蛋白的电子设备，最近克隆的荧光蛋白基因可能有助于对环境中的转基因生物进行生态监测。因此，本综述还着重讨论了生物的体内标记，尤其是植物。
