全蛋白发色团技术的最新进展。

Recent advances in all-protein chromophore technology.

作者信息

Prescott Mark, Battad Jion M, Wilmann Pascal G, Rossjohn Jamie, Devenish Rodney J

机构信息

Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University, Clayton, Victoria 3800, Australia.

出版信息

Biotechnol Annu Rev. 2006;12:31-66. doi: 10.1016/S1387-2656(06)12002-5.

DOI:10.1016/S1387-2656(06)12002-5

PMID:17045191

Abstract

The green fluorescent protein (GFP) is the foundation of a powerful technology that has revolutionized the way in which the life scientist carries out experiments in the living cell. The technology is continually evolving and improving through the development of existing proteins and discovery of new members of the all-protein chromophore (APC) family. This review gives an overview of the more recent advances in the technology with a particular focus on APCs having optical properties that are significantly red-shifted relative to those variants derived from Aequorea victoria GFP.

摘要

绿色荧光蛋白（GFP）是一项强大技术的基础，这项技术彻底改变了生命科学家在活细胞中进行实验的方式。通过现有蛋白质的开发和全蛋白发色团（APC）家族新成员的发现，该技术在不断发展和改进。本综述概述了该技术的最新进展，特别关注那些光学性质相对于源自维多利亚水母GFP的变体有显著红移的APC。