Ferreira Gregorio Ana P, Da Silva Ingrid R, Sedarati Mohammad R, Hedger John N
Fungal Biotechnology Group, School of Biosciences, University of Westminster, 115 New Cavendish Street, London W1W 6UW, UK.
Mycol Res. 2006 Feb;110(Pt 2):161-8. doi: 10.1016/j.mycres.2005.10.002.
During the interaction of two tropical agaric fungi, Marasmius pallescens and Marasmiellus troyanus, on agar media, initial deadlock between the two mycelia was ultimately followed by take-over by M. troyanus. When shaken liquid cultures of these two fungi were mixed, a rapid increase in laccase and manganese peroxidase activity, but no lignin peroxidase, was detected in the culture supernatant. Even more rapid and elevated induction of laccase occurred when filter-sterilized supernatant of Marasmius pallescens was added to Marasmiellus troyanus cultures, but the reciprocal experiment (addition of M. troyanus supernatant to M. pallescens cultures), did not lead to any increase in laccase activity. Addition of autoclaved supernatant of M. pallescens also induced laccase activity from M. troyanus cultures, but over a period of days rather than hours. Although both M. troyanus, and to a lesser extent M. pallescens, are able to produce laccases in shaken liquid culture following addition of the inducer 2,5-dimethylalanine, these experiments suggest that the presence of heat-stable and heat-labile laccase inducers secreted by M. pallescens mycelia lead to induction of laccases by M. troyanus.
在两种热带伞菌——浅黄小皮伞（Marasmius pallescens）和特罗亚小皮伞菌（Marasmiellus troyanus）在琼脂培养基上相互作用的过程中，两种菌丝体最初的僵持最终以特罗亚小皮伞菌的接管告终。当将这两种真菌的振荡液体培养物混合时，在培养上清液中检测到漆酶和锰过氧化物酶活性迅速增加，但未检测到木质素过氧化物酶。当将经过滤除菌的浅黄小皮伞上清液添加到特罗亚小皮伞菌培养物中时，漆酶的诱导作用甚至更快且更强，但反向实验（将特罗亚小皮伞菌上清液添加到浅黄小皮伞培养物中）并未导致漆酶活性增加。添加经高压灭菌的浅黄小皮伞上清液也能诱导特罗亚小皮伞菌培养物中的漆酶活性，但需要数天时间而非数小时。尽管特罗亚小皮伞菌以及程度稍轻的浅黄小皮伞菌在添加诱导剂2,5 - 二甲基丙氨酸后能够在振荡液体培养中产生漆酶，但这些实验表明，浅黄小皮伞菌菌丝体分泌的热稳定和热不稳定漆酶诱导剂的存在会导致特罗亚小皮伞菌诱导产生漆酶。
