College of Environmental Science and Engineering, Hunan University, Changsha, 410082, People's Republic of China,
Environ Sci Pollut Res Int. 2015 Jul;22(13):9807-15. doi: 10.1007/s11356-015-4149-8. Epub 2015 Feb 3.
This study was conducted to investigate the biodegradation ability of the mixed culture of Trichoderma viride and Aspergillus niger through the study of the organic matter extracted from rice straw and the lignocellulose structure by using gas chromatography-mass spectrometer (GC-MS) and Fourier transform infrared spectroscopy (FTIR). The results of the GC-MS showed that the mixed culture possessed shorter alkane (heptane) at the end of the incubation and more kinds of organic matter (except the alkanes, 29 kinds of organic matter were detected) than the pure cultures. It could be deduced that the organic matter could indicate the degradation degree of the lignocellulose to some extent. Moreover, pinene was detected in the mixed culture on days 5 and 10, which might represent the antagonistic relationship between T. viride and A. niger. The analysis of FTIR spectrums which indirectly verified the GC-MS results showed that the mixed culture possessed a better degradation of rice straw compared with the pure culture. Therefore, the methods used in this research could be considered as effective ones to investigate the lignocellulose degradation mechanism in mixed culture.
本研究通过气相色谱-质谱联用仪（GC-MS）和傅里叶变换红外光谱仪（FTIR）研究从稻草中提取的有机物和木质纤维素结构，以探讨绿色木霉和黑曲霉混合培养物的生物降解能力。GC-MS 的结果表明，混合培养物在培养结束时具有较短的烷烃（庚烷），并且比纯培养物具有更多种类的有机物（除了烷烃，还检测到 29 种有机物）。可以推断，有机物在某种程度上可以指示木质纤维素的降解程度。此外，在第 5 天和第 10 天的混合培养物中检测到了蒎烯，这可能代表了绿色木霉和黑曲霉之间的拮抗关系。FTIR 光谱分析间接验证了 GC-MS 的结果，表明混合培养物对稻草的降解能力优于纯培养物。因此，本研究中使用的方法可以被认为是研究混合培养物中木质纤维素降解机制的有效方法。
