从受不同工业源污染的废水中分离出的耐镍菌（NiRB）的研究。
The study of Nickel Resistant Bacteria (NiRB) isolated from wastewaters polluted with different industrial sources.
机构信息
Basic Medical Sciences Department, Khorasgan (Isfahan) Branch, Islamic Azad University, Isfahan, Iran.
出版信息
J Environ Health Sci Eng. 2014 Jan 29;12(1):44. doi: 10.1186/2052-336X-12-44.
BACKGROUND
Pollution due to the heavy metals is a problem that may have negative consequences on the hydrosphere. One of the best procedures in removing the toxic metals from the environment is using metal resistant bacteria.
RESULTS
In the present study eight nickel resistant bacteria were isolated from industrial wastewaters. Three of them were selected as the most resistant based on their Maximum tolerable concentration (8, 16 and 24 mM Ni2+). Their identification was done according to morphological, biochemical characteristics and 16SrDNA gene sequencing and they were identified as Cupriavidus sp ATHA3, Klebsiella oxytoca ATHA6 and Methylobacterium sp ATHA7. The accession numbers assigned to ATHA3, ATHA6 and ATHA7 strains are JX120152, JX196648 and JX457333 respectively. The Growth rate of the most resistant isolate, Klebsiella oxytoca strain ATHA6, in the presence of Ni2+ and the reduction in Ni2+ concentration was revealed that K oxytoca ATHA6 could decrease 83 mg/mL of nickel from the medium after 3 days.
CONCLUSION
It can be concluded that the identified Ni resistant bacteria could be valuable for the bioremediation of Ni polluted waste water and sewage.
背景
重金属污染是一个可能对水圈产生负面影响的问题。从环境中去除有毒金属的最佳方法之一是使用耐金属的细菌。
结果
本研究从工业废水中分离出 8 株耐镍细菌。根据其最大耐受浓度（8、16 和 24mM Ni2+），选择其中 3 株作为最耐镍的细菌。根据形态学、生化特性和 16SrDNA 基因测序对它们进行鉴定，它们被鉴定为 Cupriavidus sp ATHA3、Klebsiella oxytoca ATHA6 和 Methylobacterium sp ATHA7。分别分配给 ATHA3、ATHA6 和 ATHA7 菌株的注册号为 JX120152、JX196648 和 JX457333。在 Ni2+存在下最耐镍的分离株，Klebsiella oxytoca 菌株 ATHA6 的生长率和 Ni2+浓度的降低表明，K oxytoca ATHA6 可以在 3 天后从培养基中去除 83mg/mL 的镍。
结论
可以得出结论，鉴定出的耐镍细菌可能对受 Ni 污染的废水和污水的生物修复具有价值。
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