• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

通过等密度梯度法纯化风疹病毒：连续Percoll法与不连续蔗糖法的比较

Purification of rubella virus by isopycnic gradients: continuous Percoll versus discontinuous sucrose.

作者信息

Bustos J, Zamora P, Mejía E, Varela Y, Gómez B

机构信息

Laboratorio de Virología, Edificio A Segundo Piso, Facultad de Medicina, Universidad Nacional Autónoma de México, México City.

出版信息

Arch Virol. 1991;118(3-4):285-8. doi: 10.1007/BF01314039.

DOI:10.1007/BF01314039
PMID:1648898
Abstract

Concentrated viral suspensions were purified by isopycnic gradients: discontinuous sucrose, continuous Percoll. With Percoll, homogenous buoyant density virus were obtained; hemagglutinating titer, concentration factor, specific activity, and reproducible yield were higher than with sucrose.

摘要

浓缩病毒悬液通过等密度梯度进行纯化

不连续蔗糖梯度、连续Percoll梯度。使用Percoll时，可获得浮力密度均一的病毒；其血凝滴度、浓缩系数、比活性和可重复产量均高于使用蔗糖时。

相似文献

1
Purification of rubella virus by isopycnic gradients: continuous Percoll versus discontinuous sucrose.通过等密度梯度法纯化风疹病毒：连续Percoll法与不连续蔗糖法的比较
Arch Virol. 1991;118(3-4):285-8. doi: 10.1007/BF01314039.
2
Adjustment of the osmolality of Percoll for the isopycnic separation of cells and cell organelles.用于细胞和细胞器等密度分离的Percoll渗透压摩尔浓度的调整。
Anal Biochem. 1984 Sep;141(2):322-8. doi: 10.1016/0003-2697(84)90049-6.
3
Purification of plant mitochondria by isopycnic centrifugation in density gradients of Percoll.通过在Percoll密度梯度中进行等密度离心法纯化植物线粒体。
Arch Biochem Biophys. 1982 Aug;217(1):312-23. doi: 10.1016/0003-9861(82)90507-0.
4
Three-step isolation of human blood monocytes using discontinuous density gradients of Percoll.使用不连续的Percoll密度梯度三步分离人血单核细胞。
J Immunol Methods. 1981;41(2):225-33. doi: 10.1016/0022-1759(81)90245-3.
5
Purification of rabbit blood basophils by isopycnic banding on Percoll.通过在Percoll上进行等密度梯度离心法纯化兔血嗜碱性粒细胞。
In Vivo. 1993 Jan-Feb;7(1):31-5.
6
Isolation of Cryptosporidium oocysts and sporozoites using discontinuous sucrose and isopycnic Percoll gradients.使用不连续蔗糖和等密度 Percoll 梯度分离隐孢子虫卵囊和子孢子。
J Parasitol. 1987 Apr;73(2):314-9.
7
Concentration and purification of rubella virus hemagglutinin by hollow fiber ultrafiltration and sucrose density centrifugation.
Can J Microbiol. 1980 Nov;26(11):1334-9. doi: 10.1139/m80-221.
8
The applicability of freeze-thaw Percoll gradients to whole-cell isopycnic fractionations: preliminary results.冻融Percoll梯度在全细胞等密度分级分离中的适用性：初步结果
J Immunol Methods. 1980;37(3-4):353-61. doi: 10.1016/0022-1759(80)90321-x.
9
Purification of human blood basophils by single step isopycnic banding on Percoll.通过在Percoll上进行一步等密度梯度离心法纯化人血嗜碱性粒细胞。
J Leukoc Biol. 1984 Feb;35(2):169-77. doi: 10.1002/jlb.35.2.169.
10
A rapid method for the isolation of metastasizing tumour cells from internal organs with the help of isopycnic density-gradient centrifugation in Percoll.一种借助Percoll等密度梯度离心从内部器官中分离转移肿瘤细胞的快速方法。
Br J Cancer. 1981 Sep;44(3):356-62. doi: 10.1038/bjc.1981.192.

本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
The purification of rubella virus (RV) and determination of its polypeptide composition.风疹病毒（RV）的纯化及其多肽组成的测定。
Virology. 1981 Jan 30;108(2):491-8. doi: 10.1016/0042-6822(81)90456-6.
3
Concentration and purification of rubella virus hemagglutinin by hollow fiber ultrafiltration and sucrose density centrifugation.
Can J Microbiol. 1980 Nov;26(11):1334-9. doi: 10.1139/m80-221.
4
A method for quantitation of protein in the presence of Percoll.一种在存在聚蔗糖-泛影葡胺的情况下定量蛋白质的方法。
Anal Biochem. 1981 Oct;117(1):108-12. doi: 10.1016/0003-2697(81)90699-0.
5
Density gradient ultracentrifugation of rubella virus.
Proc Soc Exp Biol Med. 1968 Apr;127(4):1231-6. doi: 10.3181/00379727-127-32917.
6
Purification of rubella virus particles.风疹病毒颗粒的纯化
J Gen Virol. 1969 Jul;5(1):39-46. doi: 10.1099/0022-1317-5-1-39.
7
Equine abortion (herpes) virus: purification and concentration of enveloped and deenveloped virus and envelope material by density gradient centrifugation in colloidal silica.马流产（疱疹）病毒：通过在胶体二氧化硅中进行密度梯度离心法纯化和浓缩包膜病毒、脱包膜病毒及包膜物质。
Virology. 1972 May;48(2):618-23. doi: 10.1016/0042-6822(72)90077-3.
8
Density gradient centrifugation of a herpesvirus (IBRV) in colloidal silica.
Virology. 1970 Jun;41(2):368-72. doi: 10.1016/0042-6822(70)90090-5.
9
Quantitation of rubella virus by competitive enzyme immunosorbent assay.通过竞争性酶免疫吸附测定法对风疹病毒进行定量分析。
J Virol Methods. 1988 Jan;19(1):79-87. doi: 10.1016/0166-0934(88)90009-2.