通过等密度梯度法纯化风疹病毒：连续Percoll法与不连续蔗糖法的比较
Purification of rubella virus by isopycnic gradients: continuous Percoll versus discontinuous sucrose.
作者信息
Bustos J, Zamora P, Mejía E, Varela Y, Gómez B
机构信息
Laboratorio de Virología, Edificio A Segundo Piso, Facultad de Medicina, Universidad Nacional Autónoma de México, México City.
出版信息
Arch Virol. 1991;118(3-4):285-8. doi: 10.1007/BF01314039.
PMID:1648898
Abstract
Concentrated viral suspensions were purified by isopycnic gradients: discontinuous sucrose, continuous Percoll. With Percoll, homogenous buoyant density virus were obtained; hemagglutinating titer, concentration factor, specific activity, and reproducible yield were higher than with sucrose.
摘要
浓缩病毒悬液通过等密度梯度进行纯化
不连续蔗糖梯度、连续Percoll梯度。使用Percoll时，可获得浮力密度均一的病毒；其血凝滴度、浓缩系数、比活性和可重复产量均高于使用蔗糖时。
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