一种借助Percoll等密度梯度离心从内部器官中分离转移肿瘤细胞的快速方法。
A rapid method for the isolation of metastasizing tumour cells from internal organs with the help of isopycnic density-gradient centrifugation in Percoll.
作者信息
Bosslet K, Ruffmann R, Altevogt P, Schirrmacher V
出版信息
Br J Cancer. 1981 Sep;44(3):356-62. doi: 10.1038/bjc.1981.192.
Abstract
Metastasizing tumour cells from a DBA/2 mouse T-cell lymphoma could be separated from the invaded tissue by isopycnic centrifugation in continuous Percoll density gradients. The metastasizing tumour cells from spleen, liver and lung, derived from a cloned lymphoma-cell line, showed a buoyant density in Percoll of 1.060 +/- 0.010. They could be separated from the host tissue, which had a higher buoyant density in the case of the spleen cells or a lower density in the case of the dead liver or lung tissue. The separated tumour cells as removed from the gradients were viable, and could be analysed by in vitro and in vivo assays. The separation procedure did not affect the expression by the tumour cells of TATAs and H-2 antigens. Furthermore, the method seemed to be applicable to the separation of human tumour cells from mononuclear cells prepared from blood samples of tumour patients by Ficoll centrifugation.
摘要
通过在连续Percoll密度梯度中进行等密度离心，可从侵袭组织中分离出源自DBA/2小鼠T细胞淋巴瘤的转移肿瘤细胞。源自克隆淋巴瘤细胞系的脾、肝和肺中的转移肿瘤细胞，在Percoll中的漂浮密度为1.060±0.010。它们可与宿主组织分离，在脾细胞的情况下宿主组织漂浮密度较高，在肝或肺组织坏死的情况下宿主组织漂浮密度较低。从梯度中取出的分离肿瘤细胞具有活力，可通过体外和体内试验进行分析。分离过程不影响肿瘤细胞TATAs和H-2抗原的表达。此外，该方法似乎适用于从通过Ficoll离心从肿瘤患者血样制备的单核细胞中分离人肿瘤细胞。
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