Varela Y, Ortega E, Gómez B
Departamento de Ecología Humana, Facultad de Medicina, Universidad Nacional Autónoma de México, México D.F.
J Virol Methods. 1988 Jan;19(1):79-87. doi: 10.1016/0166-0934(88)90009-2.
A simple and rapid technique for quantification of rubella virus is described. The specificity of the competitive enzyme linked immunosorbent assay (ELISA) was quantified by comparing the slopes and the Y intercepts of the curves obtained when viral antigen vs. control antigen was used. The curves were derived by plotting the absorbance ratio against the logarithm of the antigen concentration. The technique was reproducible, its sensitivity depending on the purity of the antigen used. In ELISA, when an antigen precipitated by ammonium sulfate was used, the sensitivity expressed in protein/ml was 7 micrograms and when an antigen purified by sucrose gradient was used, 70 ng, whereas the limit of sensitivity in the standard technique of hemagglutination was only 38 micrograms.
本文描述了一种简单快速的风疹病毒定量技术。通过比较使用病毒抗原与对照抗原时获得的曲线斜率和Y轴截距，对竞争性酶联免疫吸附测定（ELISA）的特异性进行了定量。这些曲线是通过将吸光度比值相对于抗原浓度的对数作图得出的。该技术具有可重复性，其灵敏度取决于所用抗原的纯度。在ELISA中，当使用硫酸铵沉淀的抗原时，以蛋白质/毫升表示的灵敏度为7微克，而当使用蔗糖梯度纯化的抗原时，灵敏度为70纳克，而血凝标准技术的灵敏度极限仅为38微克。
