Balla Sudha, Thapar Vishal, Verma Snigdha, Luong Thaibinh, Faghri Tanaz, Huang Chun-Hsi, Rajasekaran Sanguthevar, del Campo Jacob J, Shinn Jessica H, Mohler William A, Maciejewski Mark W, Gryk Michael R, Piccirillo Bryan, Schiller Stanley R, Schiller Martin R
Department of Computer Science and Engineering, University of Connecticut, Storrs, Connecticut 06269, USA.
Nat Methods. 2006 Mar;3(3):175-7. doi: 10.1038/nmeth856.
In addition to large domains, many short motifs mediate functional post-translational modification of proteins as well as protein-protein interactions and protein trafficking functions. We have constructed a motif database comprising 312 unique motifs and a web-based tool for identifying motifs in proteins. Functional motifs predicted by MnM can be ranked by several approaches, and we validated these scores by analyzing thousands of confirmed examples and by confirming prediction of previously unidentified 14-3-3 motifs in EFF-1.
除了大的结构域，许多短基序介导蛋白质的功能性翻译后修饰以及蛋白质-蛋白质相互作用和蛋白质运输功能。我们构建了一个包含312个独特基序的基序数据库和一个用于识别蛋白质中基序的基于网络的工具。通过MnM预测的功能性基序可以通过几种方法进行排名，并且我们通过分析数千个已证实的例子以及通过确认对EFF-1中先前未鉴定的14-3-3基序的预测来验证这些分数。
